Shadow effects in spiral phase contrast microscopy -: art. no. 233902

被引:206
作者
Jesacher, A [1 ]
Fürhapter, S [1 ]
Bernet, S [1 ]
Ritsch-Marte, M [1 ]
机构
[1] Med Univ Innsbruck, Div Biomed Phys, A-6020 Innsbruck, Austria
关键词
D O I
10.1103/PhysRevLett.94.233902
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
Recently it has been demonstrated that spatial filtering of images in microscopy with a spiral phase element in a Fourier plane of the optical path results in a strong edge enhancement of object structures. In principle the operation is isotropic, i.e., all phase edges of a sample object are highlighted simultaneously, independent of their local direction. However, here we demonstrate that the symmetry can be broken intentionally by controlling the phase of the central area of a spiral phase hologram, which is displayed at a computer controlled spatial light modulator. This produces an apparent shadow effect which can be rotated at video rate. The resulting relieflike impression of the sample topography with a longitudinal resolution in the subwavelength regime is demonstrated by imaging a standard low contrast test sample consisting of a human cheek cell.
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页数:4
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