Target-Responsive DNA Hydrogel Mediated "Stop-Flow" Microfluidic Paper-Based Analytic Device for Rapid, Portable and Visual Detection of Multiple Targets

被引：130

作者：

Wei, Xiaofeng ^{[1
]}

Tian, Tian ^{[2
]}

Jia, Shasha ^{[2
]}

Zhu, Zhi ^{[2
]}

Ma, Yanli ^{[2
]}

Sun, Jianjun ^{[1
]}

Lin, Zhenyu ^{[1
]}

Yang, Chaoyong James ^{[2
]}

机构：

[1] Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Technol Food Sa, MOE Key Lab Anal & Detect Food Safety, Dept Chem, Fuzhou 350116, Fujian, Peoples R China

[2] Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Collaborat Innovat Ctr Chem Energy Mat, Key Lab Chem Biol Fujian Prov,MOE Key Lab Spectro, Xiamen 361005, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2015年 / 87卷 / 08期

基金：

美国国家科学基金会;

关键词：

CROSS-LINKED HYDROGEL; OF-CARE DIAGNOSTICS; POINT; PLATFORM; READOUT; CHIP; TIME; ASSAYS; LAB;

D O I：

10.1021/acs.analchem.5b00532

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A versatile point-of-care assay platform was developed for simultaneous detection of multiple targets based on a microfluidic paper-based analytic device (mu PAD) using a target-responsive hydrogel to mediate fluidic flow and signal readout. An aptamer-cross-linked hydrogel was used as a target-responsive flow regulator in the mu PAD. In the absence of a target, the hydrogel is formed in the flow channel, stopping the flow in the mu PAD and preventing the colored indicator from traveling to the final observation spot, thus yielding a signal off readout. In contrast, in the presence of a target, no hydrogel is formed because of the preferential interaction of target and aptamer. This allows free fluidic flow in the mu PAD, carrying the indicator to the observation spot and producing a signal on readout. The device is inexpensive to fabricate, easy to use, and disposable after detection. Testing results can be obtained within 6 min by the naked eye via a simple loading operation without the need for any auxiliary equipment. Multiple targets, including cocaine, adenosine, and Pb2+, can be detected simultaneously, even in complex biological matrices such as urine. The reported method offers simple, low cost, rapid, user-friendly, point-of-care testing, which will be useful in many applications.

引用

页码：4275 / 4282

页数：8

共 40 条

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