Emergence of Pathogenic Strains of Staphylococcus aureus in Goat Milk and Their Comparative Response to Antibiotics

被引：7

作者：

Muzammil, Iqra ^{[1
]}

Saleem, Muhammad Ijaz ^{[1
]}

Aqib, Amjad Islam ^{[2
]}

Ashar, Ambreen ^{[3
]}

Mahfooz, Syed Ashar ^{[1
]}

Rahman, Sajjad Ur ^{[4
]}

Shoaib, Muhammad ^{[4
]}

Naseer, Muhammad Aamir ^{[1
]}

Sohrani, Imran Khan ^{[1
]}

Ahmad, Javeed ^{[1
]}

Saqi, Razaullah ^{[1
]}

Lodhi, Fizzah Laeeq ^{[1
]}

Tanveer, Qaisar ^{[5
]}

机构：

[1] Univ Agr Faisalabad, Dept Clin Med & Surg, Fac Vet Sci, Faisalabad 38000, Pakistan

[2] Cholistan Univ Vet & Anim Sci, Dept Med, Fac Vet Sci, Bahawalpur 63100, Pakistan

[3] Govt Coll Women Univ, Faisalabad 38000, Pakistan

[4] Univ Agr Faisalabad, Inst Microbiol, Fac Vet Sci, Faisalabad 38000, Pakistan

[5] Univ Agr Faisalabad, Inst Pharm Physiol & Pharmacol, Faisalabad 38000, Pakistan

来源：

PAKISTAN JOURNAL OF ZOOLOGY | 2021年 / 53卷 / 05期

关键词：

Key words Goat; Mastitis; S; aureus; Biofilm; Risk factors; Antibiogram; ANTIMICROBIAL RESISTANCE; SUBCLINICAL MASTITIS; DRUG-RESISTANCE; PREVALENCE; COAGULASE; FARMS; EPIDEMIOLOGY; HOSPITALS; ETIOLOGY; SPP;

D O I：

10.17582/journal.pjz/20191024181008

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

The nutraceutical milk of goat in agrobased countries is at risk of contamination with pathogenic strains of Staphylococcus aureus. The current study was designed to investigate prevalence of pathogenic strains of S. aureus, assessment of risk factors, and in-vitro antibiogram of non-biofilm producing S. aureus (nbpSA) and biofilm positive S. aureus (bpSA) from mastitic goats. The purposive sampling technique was applied to collect n=200 milk samples from different regions of goat populated areas of district Faisalabad Pakistan. Using surf field mastitis test, collected milk samples were screened for subclinical mastitis at the spot for subsequent identification of pathogenic strains of S. aureus through microbiological examination in the laboratory. Non-probability statistical tools conferred 42% (84/200, CI=35.37-48.93) prevalence of subclinical mastitis, 38.1% S. aureus (32/84, CI=28.45-48.79), 15.6% MRSA (5/32, CI=6.87-31.76), 46.9% haemolytic S. aureus (15/32, CI=30.87-63.56) and 34.4 % biofilm producing S. aureus (11/32, CI=20.41-51.69). Earthen floor type (OR=1.75, p=0.0996), poor drainage system (OR=7.33, p=0.002), pond as source of drinking water (OR=2.05, p=0.179), stall feeding (OR=7.27, p<0.001), 4-6 years of age of goat (OR=4.2, p=0.0874), and teat injury (OR=13.74, p<0.001) were potential risk factors for subclinical mastitis. The in-vitro findings of current study revealed 100% sensitivity of S. aureus against gentamicin, oxytetracycline, amoxicillin, and linezolid while 80% of biofilm negative S. aureus (nbpSA) showed sensitivity against amoxicillin+clavulanic acid. None of the isolate from bpSA and nbpSA was resistant against linezolid, gentamicin, and oxytetracycline in this study. bpSA were highly resistant against amoxicillin and vancomycin. The study found higher prevalence of pathogenic strains of S. aureus, higher number of potential risk factors, and diversified responses to antibiotic.

引用

页码：1659 / 1667

页数：9