Molecular Characterization of the Cytochrome b Gene and In Vitro Atovaquone Susceptibility of Plasmodium falciparum Isolates from Kenya

被引:6
作者
Ingasia, Luicer A. [1 ]
Akala, Hoseah M. [1 ]
Imbuga, Mabel O. [2 ]
Opot, Benjamin H. [1 ]
Eyase, Fredrick L. [1 ]
Johnson, Jacob D. [1 ]
Bulimo, Wallace D. [1 ]
Kamau, Edwin [1 ]
机构
[1] Kenya Med Res Inst KEMRI Walter Reed Project, USAMRU K, Dept Emerging Infect Dis Global Emerging Infect S, Kisumu, Kenya
[2] Jomo Kenyatta Univ Agr & Technol, Coll Hlth Sci, Dept Biochem, Nairobi, Kenya
关键词
PROGUANIL TREATMENT FAILURE; RESISTANCE; MALARIA; MUTATIONS; AFRICA; POLYMORPHISMS; CONFIRMATION; EMERGENCE; PARASITES; TRAVELER;
D O I
10.1128/AAC.03956-14
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The prevalence of a genetic polymorphism(s) at codon 268 in the cytochrome b gene, which is associated with failure of atovaquone-proguanil treatment, was analyzed in 227 Plasmodium falciparum parasites from western Kenya. The prevalence of the wild-type allele was 63%, and that of the Y268S (denoting a Y-to-S change at position 268) mutant allele was 2%. There were no pure Y268C or Y268N mutant alleles, only mixtures of a mutant allele(s) with the wild type. There was a correlation between parasite 50% inhibitory concentration (IC50) and parasite genetic polymorphism; mutant alleles had higher IC(50)s than the wild type.
引用
收藏
页码:1818 / 1821
页数:4
相关论文
共 33 条
  • [1] [Anonymous], 2002, Prescrire Int, V11, P131
  • [2] [Anonymous], 1997, MMWR Recomm Rep, V46, P1
  • [3] The cost of antibiotic resistance from a bacterial perspective
    Björkman, J
    Andersson, DI
    [J]. DRUG RESISTANCE UPDATES, 2000, 3 (04) : 237 - 245
  • [4] Evaluation of atovaquone in the treatment of patients with uncomplicated Plasmodium falciparum malaria
    Chiodini, PL
    Conlon, CP
    Hutchinson, DBA
    Farquhar, JA
    Hall, AP
    Peto, TEA
    Birley, H
    Warrell, DA
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1995, 36 (06) : 1073 - 1078
  • [5] Atovaquone-proguanil treatment failure in Plasmodium folciporum
    Contentin, Laetitia
    Grammatico-Guillon, Leslie
    Desoubeaux, Guillaume
    Baron, Sabine
    Hai Duong Thanh
    [J]. PRESSE MEDICALE, 2011, 40 (11): : 1081 - 1083
  • [6] Atovaquone/proguanil resistance in Africa: a case report
    David, KP
    Alifrangis, M
    Salanti, A
    Vestergaard, LS
    Ronn, A
    Bygbjerg, I
    [J]. SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES, 2003, 35 (11-12) : 897 - 898
  • [7] Plasmodium falciparum malaria and atovaquone-proguanil treatment failure
    Durand, Remy
    Prendki, Virginie
    Cailhol, Johann
    Hubert, Vronique
    Ralaimazava, Pascal
    Massias, Laurent
    Bouchaud, Olivier
    Le Brast, Jacques
    [J]. EMERGING INFECTIOUS DISEASES, 2008, 14 (02) : 320 - 322
  • [8] The Role of Pfmdr1 and Pfcrt in Changing Chloroquine, Amodiaquine, Mefloquine and Lumefantrine Susceptibility in Western-Kenya P. falciparum Samples during 2008-2011
    Eyase, Fredrick L.
    Akala, Hoseah M.
    Ingasia, Luiser
    Cheruiyot, Agnes
    Omondi, Angela
    Okudo, Charles
    Juma, Dennis
    Yeda, Redemptah
    Andagalu, Ben
    Wanja, Elizabeth
    Kamau, Edwin
    Schnabel, David
    Bulimo, Wallace
    Waters, Norman C.
    Walsh, Douglas S.
    Johnson, Jacob D.
    [J]. PLOS ONE, 2013, 8 (05):
  • [9] Evidence of Plasmodium falciparum malaria resistant to atovaquone and proguanil hydrochloride:: case reports
    Färnert, A
    Lindberg, J
    Gil, P
    Swedberg, G
    Berqvist, Y
    Thapar, MM
    Lindegårdh, N
    Berezcky, S
    Björkman, A
    [J]. BMJ-BRITISH MEDICAL JOURNAL, 2003, 326 (7390): : 628 - 629
  • [10] Cytochrome b Mutation Y268S Conferring Atovaquone Resistance Phenotype in Malaria Parasite Results in Reduced Parasite bc1 Catalytic Turnover and Protein Expression
    Fisher, Nicholas
    Majid, Roslaini Abd
    Antoine, Thomas
    Al-Helal, Mohammed
    Warman, Ashley J.
    Johnson, David J.
    Lawrenson, Alexandre S.
    Ranson, Hilary
    O'Neill, Paul M.
    Ward, Stephen A.
    Biagini, Giancarlo A.
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (13) : 9731 - 9741