In vitro and in vivo evaluation of xenogeneic bone putty with the carrier of hydrogel derived from demineralized bone matrix

被引:8
|
作者
Zhang, Naili [1 ]
Ma, Lina [2 ]
Liu, Xiaowei [1 ]
Jiang, Xiaorui [3 ]
Yu, Zhenhai [1 ]
Zhao, Dongmei [1 ]
Zhang, Luping [1 ]
Zhang, Chunlei [1 ]
Huang, Fei [1 ]
机构
[1] Binzhou Med Univ, Sch Basic Med Sci, Dept Human Anat, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China
[2] Binzhou Med Univ, Sch Med, Dept Diagnost, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China
[3] Yuhuangding Hosp, Dept Hand & Foot Surg, 20 Yuhuangding East Rd, Yantai 264000, Peoples R China
基金
中国国家自然科学基金;
关键词
Demineralized bone matrix; Xenograft; Bone matrix gel; Bone regeneration; Moldable putty; CANCELLOUS BONE; CELL BEHAVIOR; REGENERATION; BIOCOMPATIBILITY; OSTEOINDUCTIVITY; HYDROXYAPATITE; NANOCOMPOSITE; EFFICACY;
D O I
10.1007/s10561-018-9708-z
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The demineralized bone matrix (DBM) putty is a traditional bone graft utilized to facilitate the repair and reconstruction of bone. Recent studies indicated the DBM putties with the various carriers were different in bone repairing ability. In order to prepare a kind of DBM putty with a good biocompatibility and bioactivity, the DBM gel was processed from the DBM and the feasibility as a carrier for the DBM putty was evaluated. After the bovine DBM gel was prepared, the BMPs content as well as the ability to promote osteogenic differentiation of MC3T3-E1 cells in vitro were investigated. Then the DBM putty was prepared and filled into the rat calvarial defect model to evaluate the bone repairing ability by micro-CT and histology. The result showed there was 2.953 +/- 0.054ng BMP contained in per gram of the DBM gel. And the ALP production of MC3T3-E1 cells in the DBM gels group increased with prolonged culturing, the mineralized nodules formed in MC3T3-E1 cells on 14th day after co-culture. The putty prepared by DBM gel was easy to handle without loss of DBM particles at room temperature. In the rat calvarial bone defect experiment, histological observation showed more mature bone formed in the DBM putty group than that in the type I collagen group at 12weeks, which indicated the bone putty prepared by DBM gel exhibited a better bone repair capability.
引用
收藏
页码:591 / 601
页数:11
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