Diethyl Blechnic Exhibits Anti-Inflammatory and Antioxidative Activity via the TLR4/MyD88 Signaling Pathway in LPS-Stimulated RAW264.7 Cells

被引：29
作者：
He, Jia [1 ,2 ]
Han, Shan [1 ,2 ]
Li, Xin-Xing [1 ,2 ]
Wang, Qin-Qin [1 ,2 ]
Cui, Yushun [3 ]
Chen, Yangling [1 ,2 ]
Gao, Hongwei [1 ,2 ]
Huang, Liting [1 ,2 ]
Yang, Shilin [1 ,2 ]
机构：
[1] Guangxi Univ Chinese Med, Coll Pharm, Nanning 530000, Peoples R China
[2] Guangxi Engn Technol Res Ctr Advantage Chinese Pa, Nanning 530200, Peoples R China
[3] Jiangxi Univ Tradit Chinese Med, State Key Lab Innovat Drug & Efficient Energy Sav, Nanchang 330004, Jiangxi, Peoples R China
来源：
MOLECULES | 2019年 / 24卷 / 24期
基金：
中国国家自然科学基金;
关键词：
diethyl blechnic; inflammation; TLR4/MyD88; NF-kappa B; oxidative stress; NF-KAPPA-B; OXIDATIVE STRESS; INFLAMMATION; LIPOPOLYSACCHARIDE; ACTIVATION; COX-2; INDUCTION; ALPHA; TLR4; NRF2;
D O I：
10.3390/molecules24244502
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Inflammation is a common pathogenesis in many diseases. Salvia miltiorrhiza Bunge (Danshen), a traditional Chinese medicine, has been considered to have good anti-inflammatory effects. In the present study, we investigated the anti-inflammatory effect of diethyl blechnic (DB), a novel compound isolated from Danshen, and its possible mechanisms in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. The results showed that DB can inhibit the LPS-induced pro-inflammatory cytokines release of prostaglandin E2 (PGE2) and mRNA expression of TNF-alpha, IL-6, and IL-1 beta. In addition, the results of the flow cytometry assay and the fluorometric intracellular ROS kit assay indicated that DB reduced the generation of ROS in LPS-stimualted RAW264.7 cells. DB reversed the LPS-induced loss of the mitochondrial membrane potential (MMP). Furthermore, DB suppressed the LPS-stimulated increased expression of Toll-like receptor 4 (TLR4), myeloid differential protein-88 (MyD88) and phosphorylation of TAK1, PI3K, and AKT. DB promoted NF-E2-related factor 2 (Nrf2) into the nucleus, increased the expression of heme oxygenase-1 (HO-1) and NAD(P)H dehydrogenase [quinone] 1 (NQO1) and reduced the expression of Keap1. In summary, DB may inhibit LPS-induced inflammation, which mainly occurs through TLR4/MyD88 and oxidative stress signaling pathways in RAW264.7 cells.
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页数：16
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