Toxoplasma gondii and Neospora caninum in Free-Range Chickens in Henan Province of China

被引：23

作者：

Feng, Yongjie ^{[1
]}

Lu, Yaoyao ^{[1
]}

Wang, Yinghua ^{[2
]}

Liu, Jing ^{[3
]}

Zhang, Longxian ^{[1
]}

Yang, Yurong ^{[1
]}

机构：

[1] Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450052, Peoples R China

[2] Henan Prov Ctr Anim Dis Control & Prevent, Zhengzhou 450002, Peoples R China

[3] China Agr Univ, Coll Vet Med, Beijing 100094, Peoples R China

来源：

BIOMED RESEARCH INTERNATIONAL | 2016年 / 2016卷

基金：

中国国家自然科学基金;

关键词：

GALLUS-DOMESTICUS; INFECTION; SEROPREVALENCE; PREVALENCE; ANTIBODIES; STRAINS; BRAIN; MICE; WILD; DNA;

D O I：

10.1155/2016/8290536

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background. Chickens serve as an intermediate host for Toxoplasma gondii and Neospora caninum; infection of free-range (FR) chickens with these organisms is a useful indicator of soil and environmental contamination with oocysts. A total of 700 FR chicken serum samples and 300 heart samples were collected from Henan province from March to July 2015. Antibodies to T. gondii were found in 18.86% (132/700) of the chickens by modified agglutination test (cut-off 1 : 25), while 23.14% (162/700) were positive for N. caninum by indirect fluorescent antibody test (cut-off 1 : 25). T. gondii DNA was detected in the myocardium digestion liquids of 4/25 (16%) FR chickens. The PCR results of N. caninum DNA from FR chicken myocardium digestion liquids (n = 25) were all negative. Attempts to isolate viable T. gondii were unsuccessful. The results showed that there were antibodies to T. gondii and N. caninum in FR chickens from Henan province. Accordingly, effective control of feces from cats and dogs and improved pets hygiene habits were needed. To the author's knowledge, this is the first report of the presence of N. caninum antibody in chickens from China.

引用

页数：5

共 24 条

[1]

Bártová E, 2015, FOLIA PARASIT, V62, DOI 10.14411/fp.2015.043

[2] Temporal distribution and parasite load kinetics in blood and tissues during Neospora caninum infection in mice [J].

Collantes-Fernández, E ;

López-Pérez, I ;

Alvarez-García, G ;

Ortega-Mora, LM .

INFECTION AND IMMUNITY, 2006, 74 (04) :2491-2494

[3] A review of neosporosis and pathologic findings of Neospora caninum infection in wildlife [J].

Donahoe, Shannon L. ;

Lindsay, Scott A. ;

Krockenberger, Mark ;

Phalen, David ;

Slapeta, Jan .

INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE, 2015, 4 (02) :216-238

[4] Toxoplasmosis in sentinel chickens (Gallus domesticus) in New England farms: Seroconversion, distribution of tissue cysts in brain, heart, and skeletal muscle by bioassay in mice and cats [J].

Dubey, J. P. ;

Lehmann, T. ;

Lautner, F. ;

Kwok, O. C. H. ;

Gamble, H. R. .

VETERINARY PARASITOLOGY, 2015, 214 (1-2) :55-58

[5]

Dubey J. P., 2013, CAB Reviews, V8, P1, DOI 10.1079/PAVSNNR20138055

[6] Neosporosis in animals-The last five years [J].

Dubey, J. P. ;

Schares, G. .

VETERINARY PARASITOLOGY, 2011, 180 (1-2) :90-108

[7] Toxoplasma gondii Infections in Chickens (Gallus domesticus): Prevalence, Clinical Disease, Diagnosis and Public Health Significance [J].

Dubey, J. P. .

ZOONOSES AND PUBLIC HEALTH, 2010, 57 (01) :60-73

[8]

Dubey J.P., 1988, P1

[9] A review of toxoplasmosis in wild birds [J].

Dubey, JP .

VETERINARY PARASITOLOGY, 2002, 106 (02) :121-153

[10] Sex-dependent toxoplasmosis-associated differences in testosterone concentration in humans [J].

Flegr, J. ;

Lindova, J. ;

Kodym, P. .

PARASITOLOGY, 2008, 135 (04) :427-431

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