Cell-wall associated polysaccharide from the psychrotolerant bacterium Psychrobacter arcticus 273-4: isolation, purification and structural elucidation

被引:7
作者
Casillo, Angela [1 ]
Ricciardelli, Annarita [1 ]
Parrilli, Ermenegilda [1 ]
Tutino, Maria Luisa [1 ]
Corsaro, Maria Michela [1 ]
机构
[1] Univ Napoli Federico II, Dept Chem Sci, Complesso Univ Monte S Angelo,Via Cintia, I-80126 Naples, Italy
关键词
Capsular polysaccharide; Pseudaminic acid; Extracellular polysaccharide (EPS); Psychrotolerant; CAPSULAR POLYSACCHARIDE; RHEOLOGICAL PROPERTIES; CHEMICAL-COMPOSITION; PSEUDAMINIC ACID; SEA-ICE; MARINE; EXOPOLYSACCHARIDE; TEMPERATURE; PROTEINS; SUGARS;
D O I
10.1007/s00792-019-01113-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this paper, the structure of the capsular polysaccharide isolated from the psychrotolerant bacterium Psychrobacter arcticus 273-4 is reported. The polymer was purified by gel filtration chromatography and the structure was elucidated by means of one- and two-dimensional NMR spectroscopy, in combination with chemical analyses. The polysaccharide consists of a trisaccharidic repeating unit containing two residues of glucose and a residue of a N,N-diacetyl-pseudaminic acid.
引用
收藏
页码:63 / 70
页数:8
相关论文
共 43 条
[1]   The Genome Sequence of Psychrobacter arcticus 273-4, a Psychroactive Siberian Permafrost Bacterium, Reveals Mechanisms for Adaptation to Low-Temperature Growth [J].
Ayala-del-Rio, Hector L. ;
Chain, Patrick S. ;
Grzymski, Joseph J. ;
Ponder, Monica A. ;
Ivanova, Natalia ;
Bergholz, Peter W. ;
Di Bartolo, Genevive ;
Hauser, Loren ;
Land, Miriam ;
Bakermans, Corien ;
Rodrigues, Debora ;
Klappenbach, Joel ;
Zarka, Dan ;
Larimer, Frank ;
Richardson, Paul ;
Murray, Alison ;
Thomashow, Michael ;
Tiedje, James M. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (07) :2304-2312
[2]   Relationship of critical temperature to macromolecular synthesis and growth yield in Psychrobacter cryopegella [J].
Bakermans, C ;
Nealson, KH .
JOURNAL OF BACTERIOLOGY, 2004, 186 (08) :2340-2345
[3]   Effect of growth conditions on the rheological properties and chemical composition of Volcaniella eurihalina exopolysaccharide [J].
Bejar, V ;
Calvo, C ;
Moliz, J ;
DiazMartinez, F ;
Quesada, E .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1996, 59 (01) :77-86
[4]   ISOLATION AND STRUCTURAL-ANALYSIS OF OLIGOSACCHARIDE PHOSPHATES CONTAINING THE COMPLETE CARBOHYDRATE CHAIN OF THE LIPOPOLYSACCHARIDE FROM VIBRIO-CHOLERAE STRAIN H11 (NON-O1) [J].
BOCK, K ;
VINOGRADOV, EV ;
HOLST, O ;
BRADE, H .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 225 (03) :1029-1039
[5]   LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations [J].
Braslavsky, Ido ;
Drori, Ran .
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2013, (72) :e4189
[6]   A Unique Capsular Polysaccharide Structure from the Psychrophilic Marine Bacterium Colwellia psychrerythraea 34H That Mimics Antifreeze (Glyco)proteins [J].
Carillo, Sara ;
Casillo, Angela ;
Pieretti, Giuseppina ;
Parrilli, Ermenegilda ;
Sannino, Filomena ;
Bayer-Giraldi, Maddalena ;
Cosconati, Sandro ;
Novellino, Ettore ;
Ewert, Marcela ;
Deming, Jody W. ;
Lanzetta, Rosa ;
Marino, Gennaro ;
Parrilli, Michelangelo ;
Randazzo, Antonio ;
Tutino, Maria L. ;
Corsaro, M. Michela .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (01) :179-189
[7]   Isolation, characterization and optimization of EPSs produced by a cold-adapted Marinobacter isolate from Antarctic seawater [J].
Caruso, Consolazione ;
Rizzo, Carmen ;
Mangano, Santina ;
Poli, Annarita ;
Di Donato, Paola ;
Nicolaus, Barbara ;
Finore, Ilaria ;
Di Marco, Gaetano ;
Michaud, Luigi ;
Lo Giudice, Angelina .
ANTARCTIC SCIENCE, 2019, 31 (02) :69-79
[8]   Extracellular polymeric substances with metal adsorption capacity produced by Pseudoalteromonas sp MER144 from Antarctic seawater [J].
Caruso, Consolazione ;
Rizzo, Carmen ;
Mangano, Santina ;
Poli, Annarita ;
Di Donato, Paola ;
Nicolaus, Barbara ;
Di Marco, Gaetano ;
Michaud, Luigi ;
Lo Giudice, Angelina .
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2018, 25 (05) :4667-4677
[9]   Lipid A structural characterization from the LPS of the Siberian psychro-tolerant Psychrobacter arcticus 273-4 grown at low temperature [J].
Casillo, Angela ;
Ziaco, Marcello ;
Lindner, Buko ;
Parrilli, Ermenegilda ;
Schwudke, Dominik ;
Holgado, Aurora ;
Beyaert, Rudi ;
Lanzetta, Rosa ;
Tutino, Maria Luisa ;
Corsaro, Maria Michela .
EXTREMOPHILES, 2018, 22 (06) :955-963
[10]   Exopolysaccharides from Marine and Marine Extremophilic Bacteria: Structures, Properties, Ecological Roles and Applications [J].
Casillo, Angela ;
Lanzetta, Rosa ;
Parrilli, Michelangelo ;
Corsaro, Maria Michela .
MARINE DRUGS, 2018, 16 (02)