iTRAQ Quantitative Analysis of Multidrug Resistance Mechanisms in Human Gastric Cancer Cells

被引：34
作者：
Hu, Huai-Dong [1 ]
Ye, Feng [1 ]
Zhang, Da-Zhi [1 ]
Hu, Peng [1 ]
Ren, Hong [1 ]
Li, Sang-Lin [1 ]
机构：
[1] Chongqing Med Univ, Affiliated Hosp 2, Key Lab Mol Biol Infect Dis, Minist Educ China, Chongqing 400016, Peoples R China
来源：
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2010年
基金：
中国国家自然科学基金;
关键词：
DRUG-RESISTANCE; OVARIAN-CANCER; GENE ALG-3; MASS; CARCINOMA; APOPTOSIS; OVEREXPRESSION; DISSOCIATION; SENSITIVITY; PROTEINS;
D O I：
10.1155/2010/571343
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Multidrug resistance (MDR) is a major obstacle towards a successful treatment of gastric cancer. However, the mechanisms of MDR are intricate and have not been fully understood. To elucidate the molecular mechanisms of MDR in gastric cancer, we employed the proteomic approach of isobaric tags for relative and absolute quantification (iTRAQ), followed by LC-MS/MS, using the vincristine-resistant SGC7901/VCR cell line and its parental SGC7901 cell line as a model. In total, 820 unique proteins were identified and 91 proteins showed to be differentially expressed in SGC7901/VCR compared with SGC7901. Several differentially expressed proteins were further validated by western blot analysis. Furthermore, the association of MVP, one of the highly expressed proteins in SGC7901/VCR, with MDR was verified. Our study is the first application of iTRAQ technology for MDR mechanisms analysis in gastric cancer, and many of the differentially expressed proteins identified have not been linked to MDR in gastric cancer before, which showed the value of this technology in identifying differentially expressed proteins in cancer.
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页数：11
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