Protective effect of polysaccharides from Opuntia dillenii Haw. fruits on streptozotocin-induced diabetic rats

被引：61
作者：
Gao, Jie [1 ]
Han, Yu-Lu [1 ,2 ]
Jin, Zheng-Yu [2 ]
Xu, Xue-Ming
Zha, Xue-Qiang [1 ]
Chen, Han-Qing [1 ]
Yin, Yan-Yan [3 ]
机构：
[1] Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Anhui, Peoples R China
[2] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[3] Anhui Med Univ, Dept Pharmacol, Hefei 230032, Anhui, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2015年 / 124卷
基金：
中国国家自然科学基金;
关键词：
Opuntia dillenii Haw. fruit; Polysaccharide; Antioxidant; Diabetes mellitus; Oxidative stress; INDUCED OXIDATIVE STRESS; ANTIOXIDANT ACTIVITY; ALLOXAN; MECHANISMS; COMPONENT; EXTRACTS; SYSTEM; CELLS; ASSAY;
D O I：
10.1016/j.carbpol.2015.01.068
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
In this study, a novel water-soluble polysaccharide fraction with molecular weight of 6479.1 kDa was isolated from the fruits of Opuntia dillenii Haw., which consisted of rhamnose, xylose, mannose and glucose in the molar ratio of 14.99:1.14:1.00:6.47. The protective effect of O. dillenii Haw. fruits polysaccharide (ODFP) against oxidative damage in streptozotocin (STZ)-induced diabetic rats was investigated. The results showed that oral administration of ODFP significantly decreased food intake, water intake, urine production, organ weights and blood glucose level, and increased body weight in STZ-induced diabetic rats. ODFP also significantly increased the activities of SOD, GPx and CAT, and decreased malondialdehyde level in serum, liver, kidney, and pancreas in STZ-induced diabetic rats. Moreover, histopathological examination showed that ODFP could markedly improve the structure integrity of pancreatic islet tissue in STZ-induced diabetic rats. These results suggest that ODFP have hypoglycemic and antioxidant properties and can protect rats from STZ-induced oxidative damage. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：25 / 34
页数：10
相关论文
共 40 条
[1]   Anti-diabetic and hypolipidaemic properties of ginger (Zingiber officinale) in streptozotocin-induced diabetic rats [J].
Al-Amin, Zainab M. ;
Thomson, Martha ;
Al-Qattan, Khaled K. ;
Peltonen-Shalaby, Riitta ;
Ali, Muslim .
BRITISH JOURNAL OF NUTRITION, 2006, 96 (04) :660-666
[2]   Hypoglycemic effect of extracts and fractions from Psacalium decompositum in healthy and alloxan-diabetic mice [J].
Alarcon-Aguilar, FJ ;
Jimenez-Estrada, M ;
Reyes-Chilpa, R ;
Roman-Ramos, R .
JOURNAL OF ETHNOPHARMACOLOGY, 2000, 72 (1-2) :21-27
[3]  
ALBERSHEIM PETER, 1967, CARBOHYD RES, V5, P340, DOI 10.1016/S0008-6215(00)80510-8
[4]   Beneficial effects of Murraya koenigii leaves on antioxidant defense system and ultra structural changes of pancreatic β-cells in experimental diabetes in rats [J].
Arulselvan, Palanisamy ;
Subramanian, Sorimuthu Pillai .
CHEMICO-BIOLOGICAL INTERACTIONS, 2007, 165 (02) :155-164
[5]   ROLE OF OXIDATIVE STRESS IN DEVELOPMENT OF COMPLICATIONS IN DIABETES [J].
BAYNES, JW .
DIABETES, 1991, 40 (04) :405-412
[6]  
BEERS RF, 1952, J BIOL CHEM, V195, P133
[7]   NEW METHOD FOR QUANTITATIVE-DETERMINATION OF URONIC ACIDS [J].
BLUMENKR.N ;
ASBOEHAN.G .
ANALYTICAL BIOCHEMISTRY, 1973, 54 (02) :484-489
[8]   Components and antioxidant activity of polysaccharide conjugate from green tea [J].
Chen, HX ;
Zhang, M ;
Xie, BJ .
FOOD CHEMISTRY, 2005, 90 (1-2) :17-21
[9]   Protective effect of the polysaccharide from Ophiopogon japonicus on streptozotocin-induced diabetic rats [J].
Chen, Xiaoming ;
Tang, Jia ;
Xie, Wenyuan ;
Wang, Junjun ;
Jin, Jing ;
Ren, Jun ;
Jin, Liqin ;
Lu, Jianxin .
CARBOHYDRATE POLYMERS, 2013, 94 (01) :378-385
[10]   A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].
DUBOIS, M ;
GILLES, K ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
NATURE, 1951, 168 (4265) :167-167
1234