Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review

被引:278
作者
Javier Ruiz-Ojeda, Francisco [1 ,2 ]
Iris Ruperez, Azahara [1 ]
Gomez-Llorente, Carolina [1 ,2 ,3 ]
Gil, Angel [1 ,2 ,3 ]
Maria Aguilera, Concepcion [1 ,2 ,3 ]
机构
[1] Univ Granada, Dept Biochem & Mol Biol 2, Inst Nutr & Food Technol Jose Mataix, Sch Pharm,Ctr Biomed Res, Campus Cartuja S-N,Ave Conocimiento S-N,18016, E-18071 Granada, Spain
[2] Univ Granada, Inst Invest Biosanitaria Ibs, Complejo Hosp Univ Granada, E-18014 Granada, Spain
[3] Inst Salud Carlos III, CIBEROBN Physiopathol Obes & Nutr CB12 03 30038, Madrid 28029, Spain
关键词
adipocytes; beige cells; brown adipose tissue; cell culture techniques; cell differentiation; in vitro techniques; obesity; white adipose tissue; BROWN ADIPOSE-TISSUE; INHIBITS ADIPOCYTE DIFFERENTIATION; MESENCHYMAL STEM-CELLS; MORPHOGENETIC PROTEIN 4; HUMAN PRE-ADIPOCYTES; HUMAN PREADIPOCYTES; WHITE FAT; PORCINE PREADIPOCYTES; GENE-EXPRESSION; 3T3-L1; CELLS;
D O I
10.3390/ijms17071040
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Over the last several years, the increasing prevalence of obesity has favored an intense study of adipose tissue biology and the precise mechanisms involved in adipocyte differentiation and adipogenesis. Adipocyte commitment and differentiation are complex processes, which can be investigated thanks to the development of diverse in vitro cell models and molecular biology techniques that allow for a better understanding of adipogenesis and adipocyte dysfunction associated with obesity. The aim of the present work was to update the different animal and human cell culture models available for studying the in vitro adipogenic differentiation process related to obesity and its co-morbidities. The main characteristics, new protocols, and applications of the cell models used to study the adipogenesis in the last five years have been extensively revised. Moreover, we depict co-cultures and three-dimensional cultures, given their utility to understand the connections between adipocytes and their surrounding cells in adipose tissue.
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页数:26
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