One-step reverse transcription loop-mediated isothermal amplification assay for rapid detection of Cymbidium mosaic virus

被引：49

作者：

Lee, Meng-Shiou ^{[1
]}

Yang, Meng-Ja ^{[1
]}

Hseu, You-Cheng ^{[2
]}

Lai, Guan-Hua ^{[1
]}

Chang, Wen-Te ^{[1
]}

Hsu, Yau-Heiu ^{[3
]}

Lin, Ming-Kuem ^{[1
]}

机构：

[1] China Med Univ, Sch Chinese Pharmaceut Sci & Chinese Med Resource, Taichung 40402, Taiwan

[2] China Med Univ, Coll Pharm, Dept Cosmeceut, Taichung 40402, Taiwan

[3] Natl Chung Hsing Univ, Grad Inst Biotechnol, Taichung 40227, Taiwan

来源：

JOURNAL OF VIROLOGICAL METHODS | 2011年 / 173卷 / 01期

关键词：

CymMV; RT-LAMP; Orchid; Detection; ODONTOGLOSSUM RINGSPOT TOBAMOVIRUS; 2 ORCHID VIRUSES; RT-LAMP; POTEXVIRUS; PCR; MOVEMENT;

D O I：

10.1016/j.jviromet.2011.01.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cymbidium mosaic virus (CymMV) is the most prevalent orchid virus. A single-tube one-step betaine-free reverse transcription (RI) loop-mediated isothermal amplification (LAMP) assay was developed for the rapid and easy detection of orchid-infecting CymMV. Five sets of primers were designed based on the conserved regions among various virus isolates. The specificity and the sensitivity of the assay were then evaluated using the RI-LAMP reaction. Within 1 h under isothermal conditions at 60 degrees C the target viral gene was amplified successfully. This RT-LAMP assay was found to be quick, specific, sensitive and easy to perform assay that involved only one step and was simpler to carry out than alternative approaches. Thus this assay is an alternative for the rapid and easy detection of CymMV in orchids. This is first time that a RI-LAMP method for the detection of an orchid virus has been described. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：43 / 48

页数：6

共 19 条

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