Infection and Rapid Transmission of SARS-CoV-2 in Ferrets

被引：680

作者：

Kim, Young-Il ^{[1
,2
,3
]}

Kim, Seong-Gyu ^{[1
,2
]}

Kim, Se-Mi ^{[1
,2
]}

Kim, Eun-Ha ^{[1
,2
,3
]}

Park, Su-Jin ^{[1
,2
,3
]}

Yu, Kwang-Min ^{[1
,2
,3
]}

Chang, Jae-Hyung ^{[1
,2
]}

Kim, Eun Ji ^{[1
,2
]}

Lee, Seunghun ^{[1
,2
]}

Casel, Mark Anthony B. ^{[1
,2
,3
]}

Um, Jihye ^{[5
]}

Song, Min-Suk ^{[1
,2
,3
]}

Jeong, Hye Won ^{[1
,2
]}

Lai, Van Dam ^{[4
]}

Kim, Yeonjae ^{[5
]}

Chin, Bum Sik ^{[5
]}

Park, Jun-Sun ^{[5
]}

Chung, Ki-Hyun ^{[5
]}

Foo, Suan-Sin ^{[6
]}

Poo, Haryoung ^{[7
]}

Mo, In-Pil ^{[4
]}

Lee, Ok-Jun ^{[1
,2
]}

Webby, Richard J. ^{[8
]}

Jung, Jae U. ^{[6
]}

Choi, Young Ki ^{[1
,2
,3
]}

机构：

[1] Chungbuk Natl Univ, Coll Med, Cheongju, South Korea

[2] Chungbuk Natl Univ, Med Res Inst, Cheongju, South Korea

[3] Chungbuk Natl Univ, Zoonot Infect Dis Res Ctr, Cheongju, South Korea

[4] Chungbuk Natl Univ, Coll Vet Med, Cheongju, South Korea

[5] Natl Med Ctr, Res Inst Publ Hlth, Seoul, South Korea

[6] Univ Southern Calif, Keck Sch Med, Dept Mol Microbiol & Immunol, Los Angeles, CA 90033 USA

[7] Univ Sci & Technol, Infect Dis Res Ctr, Korea Res Inst Biosci & Biotechnol, Daejeon, South Korea

[8] St Jude Childrens Res Hosp, Dept Infect Dis, Div Virol, Memphis, TN 38105 USA

来源：

CELL HOST & MICROBE | 2020年 / 27卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

RESPIRATORY SYNDROME SARS; CORONAVIRUS; VIRUS; MODELS;

D O I：

10.1016/j.chom.2020.03.023

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in China and rapidly spread worldwide. To prevent SARS-CoV-2 dissemination, understanding the in vivo characteristics of SARS-CoV-2 is a high priority. We report a ferret model of SARS-CoV-2 infection and transmission that recapitulates aspects of human disease. SARS-CoV-2-infected ferrets exhibit elevated body temperatures and virus replication. Although fatalities were not observed, SARS-CoV-2-infected ferrets shed virus in nasal washes, saliva, urine, and feces up to 8 days post-infection. At 2 days post-contact, SARS-CoV-2 was detected in all naive direct contact ferrets. Furthermore, a few naive indirect contact ferrets were positive for viral RNA, suggesting airborne transmission. Viral antigens were detected in nasal turbinate, trachea, lungs, and intestine with acute bronchiolitis present in infected lungs. Thus, ferrets represent an infection and transmission animal model of COVID-19 that may facilitate development of SARS-CoV-2 therapeutics and vaccines.

引用

页码：704 / +

页数：8

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