Comparison of separation performance of laser-ablated and wet-etched microfluidic devices

被引:20
作者
Baker, Christopher A. [1 ]
Bulloch, Rayford [1 ]
Roper, Michael G. [1 ]
机构
[1] Florida State Univ, Dept Chem & Biochem, Tallahassee, FL 32306 USA
基金
美国国家卫生研究院;
关键词
Laser ablation; Electrophoresis; Microfluidic; Separation; SINGLE-MOLECULE DETECTION; CONTINUOUS-FLOW; CHIP; GLASS; SURFACE; POLY(DIMETHYLSILOXANE); ELECTROPHORESIS; MICROCHIP; SYSTEMS; PULSES;
D O I
10.1007/s00216-010-4144-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Laser ablation of glass allows for production of microfluidic devices without the need for hydrofluoric acid and photolithography. The goal of this study was to compare the separation performance of microfluidic devices produced using a low-cost laser ablation system and conventional wet etching. During laser ablation, cracking of the glass substrate was prevented by heating the glass to 300 degrees C. A range of laser energy densities was found to produce channel depths ranging from 4 to 35 mu m and channel widths from 118 to 162 mu m. The electroosmotic flow velocity was lower in laser-ablated devices, 0.110 +/- 0.005 cm s(-1), as compared to wet-etched microfluidic chips, 0.126 +/- 0.003 cm s(-1). Separations of both small and large molecules performed on both wet- and laser-ablated devices were compared by examining limits of detection, theoretical plate count, and peak asymmetry. Laser-induced fluorescence detection limits were 10 pM fluorescein for both types of devices. Laser-ablated and wet-etched microfluidic chips had reproducible migration times with <= 2.8% relative standard deviation and peak asymmetries ranged from 1.0 to 1.8. Numbers of theoretical plates were between 2.8- and 6.2-fold higher on the wet-etched devices compared to laser-ablated devices. Nevertheless, resolution between small and large analytes was accomplished, which indicates that laser ablation may find an application in pedagogical studies of electrophoresis or microfluidic devices, or in settings where hydrofluoric acid cannot be used.
引用
收藏
页码:1473 / 1479
页数:7
相关论文
共 28 条
[1]   A continuous-flow, microfluidic fraction collection device [J].
Baker, Christopher A. ;
Roper, Michael G. .
JOURNAL OF CHROMATOGRAPHY A, 2010, 1217 (28) :4743-4748
[2]   Microchip-based purification of DNA from biological samples [J].
Breadmore, MC ;
Wolfe, KA ;
Arcibal, IG ;
Leung, WK ;
Dickson, D ;
Giordano, BC ;
Power, ME ;
Ferrance, JP ;
Feldman, SH ;
Norris, PM ;
Landers, JP .
ANALYTICAL CHEMISTRY, 2003, 75 (08) :1880-1886
[3]   The chemistrode: A droplet-based microfluidic device for stimulation and recording with high temporal, spatial, and chemical resolution [J].
Chen, Delai ;
Du, Wenbin ;
Liu, Ying ;
Liu, Weishan ;
Kuznetsov, Andrey ;
Mendez, Felipe E. ;
Philipson, Louis H. ;
Ismagilov, Rustem F. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (44) :16843-16848
[4]   Rapid prototyping of microfluidic systems in poly(dimethylsiloxane) [J].
Duffy, DC ;
McDonald, JC ;
Schueller, OJA ;
Whitesides, GM .
ANALYTICAL CHEMISTRY, 1998, 70 (23) :4974-4984
[5]   DNA fragment sizing by single molecule detection in submicrometer-sized closed fluidic channels [J].
Foquet, M ;
Korlach, J ;
Zipfel, W ;
Webb, WW ;
Craighead, HG .
ANALYTICAL CHEMISTRY, 2002, 74 (06) :1415-1422
[6]   Integrated microfluidic system enabling protein digestion, peptide separation, and protein identification [J].
Gao, J ;
Xu, JD ;
Locascio, LE ;
Lee, CS .
ANALYTICAL CHEMISTRY, 2001, 73 (11) :2648-2655
[7]   MICROMACHINING A MINIATURIZED CAPILLARY ELECTROPHORESIS-BASED CHEMICAL-ANALYSIS SYSTEM ON A CHIP [J].
HARRISON, DJ ;
FLURI, K ;
SEILER, K ;
FAN, ZH ;
EFFENHAUSER, CS ;
MANZ, A .
SCIENCE, 1993, 261 (5123) :895-897
[8]   A rapid diffusion immunoassay in a T-sensor [J].
Hatch, A ;
Kamholz, AE ;
Hawkins, KR ;
Munson, MS ;
Schilling, EA ;
Weigl, BH ;
Yager, P .
NATURE BIOTECHNOLOGY, 2001, 19 (05) :461-465
[9]   Multiplexed Real-Time Polymerase Chain Reaction on a Digital Microfluidic Platform [J].
Hua, Zhishan ;
Rouse, Jeremy L. ;
Eckhardt, Allen E. ;
Srinivasan, Vijay ;
Pamula, Vamsee K. ;
Schell, Wiley A. ;
Benton, Jonathan L. ;
Mitchell, Thomas G. ;
Pollack, Michael G. .
ANALYTICAL CHEMISTRY, 2010, 82 (06) :2310-2316
[10]   CURRENT-MONITORING METHOD FOR MEASURING THE ELECTROOSMOTIC FLOW-RATE IN CAPILLARY ZONE ELECTROPHORESIS [J].
HUANG, XH ;
GORDON, MJ ;
ZARE, RN .
ANALYTICAL CHEMISTRY, 1988, 60 (17) :1837-1838