A high performance test of differential gene expression for oligonucleotide arrays

被引：49
作者：
Lemon, WJ
Liyanarachchi, S
You, M
机构：
[1] Washington Univ, Dept Surg, St Louis, MO 63110 USA
[2] Ohio State Univ, Ctr Comprehens Canc, Div Human Canc Genet, Columbus, OH 43210 USA
来源：
GENOME BIOLOGY | 2003年 / 4卷 / 10期
关键词：
D O I：
10.1186/gb-2003-4-10-r67
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Logit-t employs a logit-transformation for normalization followed by statistical testing at the probe-level. Using four publicly-available datasets, together providing 2,710 known positive incidences of differential expression and 2,913,813 known negative incidences, performance of statistical tests were: Logit-t provided 75% positive-predictive value, compared with 5% for Affymetrix Microarray Suite 5, 6% for dChip perfect match (PM)-only, and 9% for Robust Multi-array Analysis at the p < 0.01 threshold. Logit-t provided 70% sensitivity, Microarray Suite 5 provided 46%, dChip provided 53% and Robust Multi-array Analysis provided 63%.
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页数：11
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