Sources of scattering in cervical tissue: determination of the scattering coefficient by confocal microscopy

被引：58
作者：
Collier, T [1 ]
Follen, M
Malpica, A
Richards-Kortum, R
机构：
[1] Univ Texas, Dept Biomed Engn, Austin, TX 78712 USA
[2] Univ Texas, Dept Elect & Comp Engn, Austin, TX 78712 USA
[3] Univ Texas, MD Anderson Canc Ctr, Dept Pathol, Houston, TX 77030 USA
[4] Univ Texas, MD Anderson Canc Ctr, Dept Gynecol Oncol, Houston, TX 77030 USA
来源：
APPLIED OPTICS | 2005年 / 44卷 / 11期
关键词：
D O I：
10.1364/AO.44.002072
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Most models of light propagation through tissue assume that the scattering properties of various tissue layers are the same. We present evidence that the scattering coefficient of cervical epithelium varies by a factor of 3 within the epithelium owing to variations in nuclear density and to the presence of keratin. We estimated the scattering coefficient from regions of normal and precancerous cervical epithelium by fitting reflectance measurements from confocal images to an exponential function of depth based on Beer's law of attenuation. The results suggest that the normal cervix is characterized by highly variable scattering in the superficial epithelium, low scattering in the intermediate epithelium, and high scattering in the basal and stromal regions. In high-grade dysplasia, high scattering from high-density nuclei is observed throughout the entire epithelium. (c) 2005 Optical Society of America.
引用
收藏
页码：2072 / 2081
页数：10
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