Phytase production by the yeast, Pichia anomala

被引：65

作者：

Vohra, A ^{[1
]}

Satyanarayana, T ^{[1
]}

机构：

[1] Univ Delhi, Dept Microbiol, New Delhi 110021, India

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 07期

关键词：

phytase; Pichia anomala; yeast;

D O I：

10.1023/A:1010314114053

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pichia anomala, isolated from dried flower buds of Woodfordia fruticosa, produced a high activity of an intracellular phytase, at 68 U per g dry biomass, when grown at 20 degreesC for 24 h in a medium containing glucose (40 g l(-1)) and beef extract (10 g l(-1)) supplemented with Fe2+ (0.15 mM). Partially purified phytase was optimally active at 60 degreesC and pH 4 with a half life of 7 days at 60 degreesC. It retained 85% of its activity at 80 degreesC for 15 min. The enzyme is suitable for supplementing animal feeds to improve the availability of phosphate from phytate.

引用

页码：551 / 554

页数：4

共 12 条

[1]

BARNETT JA, 2000, YEASTS CHARACTERISTI, P494

[2]

Fiske CH, 1925, J BIOL CHEM, V66, P375

[3] Partial purification of phytase from a soil isolate bacterium, Klebsiella oxytoca MO-3 [J].