Overexpression of nicotinamidase 3 (NIC3) gene and the exogenous application of nicotinic acid (NA) enhance drought tolerance and increase biomass in Arabidopsis

被引：22

作者：

Ahmad, Zarnab ^{[1
,2
]}

Bashir, Khurram ^{[1
,3
]}

Matsui, Akihiro ^{[1
,4
]}

Tanaka, Maho ^{[1
,4
]}

Sasaki, Ryosuke ^{[5
]}

Oikawa, Akira ^{[5
,6
]}

Hirai, Masami Yokota ^{[5
,7
,8
]}

Chaomurilege ^{[9
]}

Zu, Yanhui ^{[9
]}

Kawai-Yamada, Maki ^{[9
]}

Rashid, Bushra ^{[2
]}

Husnain, Tayyab ^{[2
]}

Seki, Motoaki ^{[1
,4
,10
]}

机构：

[1] RIKEN Ctr Sustainable Resource Sci CSRS, Plant Genom Network Res Team, Yokohama, Kanagawa 2300045, Japan

[2] Univ Punjab, Ctr Excellence Mol Biol, Plant Genom Lab, Lahore, Pakistan

[3] Lahore Univ Management Sci LUMS, Syed Babar Ali Sch Sci & Engn, Dept Biol, Lab Plant Biotechnol, Lahore, Pakistan

[4] RIKEN Cluster Pioneering Res, Plant Epigenome Regulat Lab, Saitama 3510198, Japan

[5] RIKEN Ctr Sustainable Resource Sci CSRS, Mass Spectrometry & Microscopy Unit, Yokohama, Kanagawa 2300045, Japan

[6] Yamagata Univ, Fac Agr, Tsuruoka, Yamagata 9978555, Japan

[7] RIKEN Ctr Sustainable Resource Sci CSRS, Metab Syst Res Team, Yokohama, Kanagawa 2300045, Japan

[8] Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan

[9] Saitama Univ, Grad Sch Sci & Engn, Sakura Ku, 225 Shimo Okubo, Saitama 3388570, Japan

[10] Yokohama City Univ, Kihara Inst Biol Res, Yokohama, Kanagawa 2440813, Japan

来源：

PLANT MOLECULAR BIOLOGY | 2021年 / 107卷 / 1-2期

基金：

日本科学技术振兴机构;

关键词：

Nicotinamidase; 3; Drought tolerance; Nicotinic acid (NA); NAD; NAD salvage pathway; Plant growth; TRANSCRIPTION FACTOR; STRESS-RESPONSE; HIGH-SALINITY; ROOT-GROWTH; POLY(ADP-RIBOSE) POLYMERASE; SALVAGE PATHWAY; BIOSYNTHESIS; HOMEOSTASIS; DEACETYLASE; SENSITIVITY;

D O I：

10.1007/s11103-021-01179-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Key message Overexpressing Nicotinamidase 3 gene, and the exogenous application of its metabolite nicotinic acid (NA), enhance drought stress tolerance and increase biomass in Arabidopsis thaliana. With progressive global climatic changes, plant productivity is threatened severely by drought stress. Deciphering the molecular mechanisms regarding genes responsible for balancing plant growth and stress amelioration could imply multiple possibilities for future sustainable goals. Nicotinamide adenine dinucleotide (NAD) biosynthesis and recycling/ distribution is a crucial feature for plant growth. The current study focuses on the functional characterization of nicotinamidase 3 (NIC3) gene, which is involved in the biochemical conversion of nicotinamide (NAM) to nicotinic acid (NA) in the salvage pathway of NAD biosynthesis. Our data show that overexpression of NIC3 gene enhances drought stress tolerance and increases plant growth. NIC3-OX plants accumulated more NA as compared to WT plants. Moreover, the upregulation of several genes related to plant growth/stress tolerance indicates that regulating the NAD salvage pathway could significantly enhance plant growth and drought stress tolerance. The exogenous application of nicotinic acid (NA) showed a similar phenotype as the effect of overexpressing NIC3 gene. In short, we contemplated the role of NIC3 gene and NA application in drought stress tolerance and plant growth. Our results would be helpful in engineering plants with enhanced drought stress tolerance and increased growth potential.

引用

页码：63 / 84

页数：22

共 75 条

[1] Role of DREB transcription factors in abiotic and biotic stress tolerance in plants [J].