Automated dielectrophoretic cell Fractionation system using MEMS technology

被引：1

作者：

Huang, Chengjun ^{[1
,3
]}

Zhu, Jiang ^{[1
,2
]}

Wang, Lei ^{[1
,2
]}

Gu, Min ^{[2
]}

Yu, Jun ^{[1
,3
]}

Cheng, Jing ^{[1
,2
]}

机构：

[1] Huazhong Univ Sci & Technol, Dept Elect Sci & Technol, Wuhan, Peoples R China

[2] Tsinghua Univ, Sch Med, Med Syst Biol Res Ctr, Beijing, Peoples R China

[3] Natl Engn Res Ctr Beijing Biochip Technol, Beijing, Peoples R China

来源：

2006 1ST IEEE INTERNATIONAL CONFERENCE ON NANO/MICRO ENGINEERED AND MOLECULAR SYSTEMS, VOLS 1-3 | 2006年

关键词：

cell fractionation; dielectrophoresis; cell tracking;

D O I：

10.1109/NEMS.2006.334780

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A novel method for constructing an automated cell fractionation system was developed by integrating traveling-wave dielectrophoresis (twDEP) and laminar flow on a single biochip-based device. Novel electrodes designs on Indium Tin Oxide (ITO) glass and pre-molded poly(dimethylsiloxane) (PDMS) microfluidic chamber, as well as an automated cell tracking method based on a computer-assisted image analysis method were designed and developed. The functioning of the device was demonstrated by isolating viable human myelogenous HL-60 cells from the dead ones. The simulation of the electric field and fluidic field, together with the experimental results confirmed our theoretical predictions. The preliminary data achieved with this device indicated that cells of different types could be effectively separated.

引用

页码：1422 / +

页数：3

共 47 条

[41] High-throughput gene expression analysis at the level of single proteins using a microfluidic turbidostat and automated cell tracking [J].

Ullman, G. ;

Wallden, M. ;

Marklund, E. G. ;

Mahmutovic, A. ;

Razinkov, Ivan ;

Elf, J. .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2013, 368 (1611)

[42] AUTOMATED LINEAGE TREE RECONSTRUCTION FROM CAENORHABDITIS ELEGANS IMAGE DATA USING PARTICLE FILTERING BASED CELL TRACKING [J].

Carranza, Noemi ;

Smal, Ihor ;

Dzyubachyk, Oleh ;

Niessen, Wiro ;

Meijering, Erik .

2011 8TH IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING: FROM NANO TO MACRO, 2011, :1921-1924

[43] Evaluation of the Correlation between Focal Adhesion Kinase Phosphorylation and Cell Adhesion Force Using "DEP" Technology [J].

Ay, Chyung ;

Yeh, Chih-Chang ;

Hsu, Min-Chih ;

Hurng, Huaang-Youh ;

Kwok, Philip Chi Lip ;

Chang, Hsin-I. .

SENSORS, 2012, 12 (05) :5951-5965

[44] CONSTRUCTION OF A YEAST-CELL FUSION SYSTEM USING A FLUID INTEGRATED-CIRCUIT [J].

URANO, N ;

KAMIMURA, M ;

NANBA, T ;

OKADA, M ;

FUJIMOTO, M ;

WASHIZU, M .

JOURNAL OF BIOTECHNOLOGY, 1991, 20 (01) :109-116

[45] Analysis of red blood cell deformability using parallel ladder electrodes in a microfluidic manipulation system [J].

Wanting Li ;

Botao Zhu ;

Yifan Cai ;

Zhengtian Wu ;

Lining Sun ;

Hao Yang .

The International Journal of Advanced Manufacturing Technology, 2019, 105 :4919-4928

[46] Analysis of red blood cell deformability using parallel ladder electrodes in a microfluidic manipulation system [J].

Li, Wanting ;

Zhu, Botao ;

Cai, Yifan ;

Wu, Zhengtian ;

Sun, Lining ;

Yang, Hao .

INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY, 2019, 105 (12) :4919-4928

[47] Fabrication of a 3D carbon electrode for potential dielectrophoresis-based hepatic cell patterning application using carbon micro-electrical-mechanical system (CMEMS) [J].

Yahya, Wan Nurlina Wan ;

Ibrahim, Fatimah ;

Thiha, Aung ;

Jamaluddin, Nurul Fauzani ;

Madou, Marc .

JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2022, 32 (05)

← 1 2 3 4 5 →