A simple fluorescein derived colorimetric and fluorescent 'off-on' sensor for the detection of hypochlorite

被引：27

作者：

Jin, Lei ^{[1
,2
]}

Xu, Mengyi ^{[1
]}

Jiang, Hua ^{[1
]}

Wang, Wenling ^{[1
]}

Wang, Qingming ^{[1
]}

机构：

[1] Yancheng Teachers Univ, Sch Pharm, Jiangsu Prov Key Lab Coastal Wetland Bioresources, Yancheng 224051, Jiangsu, Peoples R China

[2] Nanjing Univ Technol, Coll Biotechnol & Pharmaceut Engn, Nanjing 210009, Jiangsu, Peoples R China

来源：

ANALYTICAL METHODS | 2018年 / 10卷 / 37期

关键词：

LIVING CELLS; SELECTIVE DETECTION; HYDROGEN-PEROXIDE; OXIDATIVE STRESS; AQUEOUS-SOLUTION; FAST-RESPONSE; PROBE; ACID; MYELOPEROXIDASE; WATER;

D O I：

10.1039/c8ay01489b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, we have successfully provided a novel strategy to probe for ClO-. The strategy is based on a specific reaction promoted by ClO-: namely, hypochlorite oxidizes the amino group in probe 1 to an imine. Interestingly, in the presence of ClO-, a colorless solution of probe 1 turned pink with a maximum absorption band at 479 nm, and a 150-fold fluorescence enhancement at 553 nm was found. Probe 1 is highly selective to ClO- among other anions with a detection limit of 0.023 M. The membrane-permeable probe 1 was successfully demonstrated for monitoring ClO- in cultured HeLa cells. Therefore, probe 1 can be used for detection in practical samples. Probe 1 developed herein makes possible the use of organic complexes containing amines for designing fluorescence probes for ClO-.

引用

页码：4562 / 4569

页数：8

共 35 条

[1] DIRECT AND SEQUENTIAL POTENTIOMETRIC DETERMINATION OF HYPOCHLORITE, CHLORITE, AND CHLORATE IONS WHEN HYPOCHLORITE ION IS PRESENT IN LARGE EXCESS [J].