Agrobacterium-mediated Transformation of Jatropha curcas Young Leaf Explants with Lateral Shoot-Inducing Factor (LIF)

Limited branching is considered as one of the major factors limiting oil-bearing seed yield in Jatropha clams L Seeking for promoting branches Lateral shoot-Inducing Factor (LIP) was going to be transferred into the J curcas genome For this transgenic approach to be successful, we developed a stable and efficient transformation protocol using Agrobacterium tumefaciens strain LBA4404 for young leaf explants of J curcas Parameters optimized were bacterial strain, bacterial concentration, co-cultivation period, immersion time and concentration of cefotaxime and kanamycin Transformation with LIF gene was achieved subsequently PCR amplification confirmed that the transformation efficiency was 23 91 +/- 5 78% Southern blot hybridization was performed to reveal that single and multiple copy events and further confirm that LIP was stably integrated into the J curcas genome (C) 2010 Friends Science Publishers