Non-thermal effects of terahertz radiation on gene expression in mouse stem cells

被引：71

作者：

Alexandrov, Boian S. ^{[1
]}

Rasmussen, Kim O. ^{[1
]}

Bishop, Alan R. ^{[1
]}

Usheva, Anny ^{[3
]}

Alexandrov, Ludmil B. ^{[2
]}

Chong, Shou ^{[3
]}

Dagon, Yossi ^{[3
]}

Booshehri, Layla G. ^{[4
]}

Mielke, Charles H. ^{[4
]}

Phipps, M. Lisa ^{[5
]}

Martinez, Jennifer S. ^{[5
]}

Chen, Hou-Tong ^{[5
]}

Rodriguez, George ^{[5
]}

机构：

[1] Los Alamos Natl Lab, Div Theoret, Los Alamos, NM 87545 USA

[2] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England

[3] Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Dept Med, Boston, MA 02215 USA

[4] Los Alamos Natl Lab, Mat Phys & Applicat Div NHMFL, Los Alamos, NM 87545 USA

[5] Los Alamos Natl Lab, Ctr Integrated Nanotechnol, Los Alamos, NM 87545 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2011年 / 2卷 / 09期

基金：

美国国家卫生研究院;

关键词：

GENERATION; DYNAMICS; WEAPONS; CANCER; MODES;

D O I：

10.1364/BOE.2.002679

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In recent years, terahertz radiation sources are increasingly being exploited in military and civil applications. However, only a few studies have so far been conducted to examine the biological effects associated with terahertz radiation. In this study, we evaluated the cellular response of mesenchymal mouse stem cells exposed to THz radiation. We apply low-power radiation from both a pulsed broad-band (centered at 10 THz) source and from a CW laser (2.52 THz) source. Modeling, empirical characterization, and monitoring techniques were applied to minimize the impact of radiation-induced increases in temperature. qRT-PCR was used to evaluate changes in the transcriptional activity of selected hyperthermic genes. We found that temperature increases were minimal, and that the differential expression of the investigated heat shock proteins (HSP105, HSP90, and CPR) was unaffected, while the expression of certain other genes (Adiponectin, GLUT4, and PPARG) showed clear effects of the THz irradiation after prolonged, broad-band exposure. (C) 2011 Optical Society of America

引用

页码：2679 / 2689

页数：11

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