Disruption of pknG enhances production of gamma-aminobutyric acid by Corynebacterium glutamicum expressing glutamate decarboxylase

被引:53
作者
Okai, Naoko [1 ]
Takahashi, Chihiro [2 ]
Hatada, Kazuki [2 ]
Ogino, Chiaki [2 ]
Kondo, Akihiko [2 ]
机构
[1] Kobe Univ, Org Adv Sci & Technol, Nada Ku, Kobe, Hyogo 6578501, Japan
[2] Kobe Univ, Grad Sch Engn, Dept Chem Sci & Engn, Nada Ku, Kobe, Hyogo 6578501, Japan
来源
AMB EXPRESS | 2014年 / 4卷
关键词
Corynebacterium glutamicum; Gamma-aminobutyric acid; Glutamate decarboxylase; 2-oxoglutarate dehydrogenase; Protein kinase G; ALPHA-AMYLASE; AMINO-ACIDS; DEGRADATION; CADAVERINE; MECHANISM; STARCH; LYSINE; ODHI;
D O I
10.1186/s13568-014-0020-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Gamma-aminobutyric acid (GABA), a building block of the biodegradable plastic polyamide 4, is synthesized from glucose by Corynebacterium glutamicum that expresses Escherichia coli glutamate decarboxylase (GAD) B encoded by gadB. This strain was engineered to produce GABA more efficiently from biomass-derived sugars. To enhance GABA production further by increasing the intracellular concentration of its precursor glutamate, we focused on engineering pknG (encoding serine/threonine protein kinase G), which controls the activity of 2-oxoglutarate dehydrogenase (Odh) in the tricarboxylic acid cycle branch point leading to glutamate synthesis. We succeeded in expressing GadB in a C. glutamicum strain harboring a deletion of pknG. C. glutamicum strains GAD and GAD Delta pknG were cultured in GP2 medium containing 100 g L-1 glucose and 0.1 mM pyridoxal 5'-phosphate. Strain GAD Delta pknG produced 31.1 +/- 0.41 g L-1 (0.259 g L-1 h(-1)) of GABA in 120 hours, representing a 2.29-fold higher level compared with GAD. The production yield of GABA from glucose by GAD Delta pknG reached 0.893 mol mol(-1).
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页码:1 / 8
页数:8
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