Revisiting Metarhizium and the description of new species from Thailand

被引：108

作者：

Mongkolsamrit, S. ^{[1
]}

Khonsanit, A. ^{[1
]}

Thanakitpipattana, D. ^{[1
]}

Tasanathai, K. ^{[1
]}

Noisripoom, W. ^{[1
]}

Lamlertthon, S. ^{[2
]}

Himaman, W. ^{[3
]}

Houbraken, J. ^{[4
]}

Samson, R. A. ^{[4
]}

Luangsa-ard, J. ^{[1
]}

机构：

[1] Natl Ctr Genet Engn & Biotechnol BIOTEC, 113 Thailand Sci Pk,Phahonyothin Rd, Khlong Luang 12120, Pathum Thani, Thailand

[2] Naresuan Univ, Fac Med Sci, Ctr Excellence Fungal Res, Phitsanulok 65000, Thailand

[3] Dept Natl Pk Wildlife & Plant Conservat, Forest Entomol & Microbiol Res Grp, Forest & Plant Conservat Res Off, 61 Phahonyothin Rd, Bangkok 10900, Thailand

[4] Westerdijk Fungal Biodivers Inst, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands

来源：

STUDIES IN MYCOLOGY | 2020年 / 95期

关键词：

Biological control; Clavicipitaceae; Entomopathogenic fungi; INSECT-PATHOGENIC FUNGI; ENTOMOPATHOGENIC FUNGUS; PHYLOGENETIC ANALYSIS; SP NOV; METARRHIZIUM-ANISOPLIAE; MULTIGENE PHYLOGENY; CORDYCEPS-GUNNII; RIBOSOMAL DNA; GENUS; DIVERSITY;

D O I：

10.1016/j.simyco.2020.04.001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Over the last two decades the molecular phylogeny and classification of Metarhizium has been widely studied. Despite these efforts to understand this enigmatic genus, the basal lineages in Metarhizium are still poorly resolved. In this study, a phylogenetic framework is reconstructed for the Clavicipitaceae focusing on Metarhizium through increased taxon-sampling using five genomic loci (SSU, LSU, tef, rpb1, rpb2) and the barcode marker ITS rDNA. Multi-gene phylogenetic analyses and morphological characterisation of green-spored entomopathogenic Metarhizium isolates from Thailand and soil isolates of M. carneum and M. marquandii reveal their ecological, genetic and species diversity. Nineteen new species are recognised in the Metarhizium clade with narrow host ranges: two new species are found in the M. anisopliae complex - M. clavatum on Coleoptera larvae and M. sulphureum on Lepidoptera larvae; four new species are found in the M. flavoviride complex M. biotecense and M. fusoideum on brown plant hoppers (Hemiptera), M. culicidarum on mosquitoes, M. nornnoi on Lepidoptera larvae; three new species M. megapomponiae, M. cicadae, M. niveum occur on cicadas; five new species M. candelabrum, M. cercopidarum, M. ellipsoideum, M. huainamdangense M. ovoidosporum occur on planthoppers, leafhoppers and froghoppers (Hemiptera); one new species M. eburneum on Lepidoptera pupae; and four new species M. phuwiangense, M. purpureum, M. purpureonigrum, M. flavum on Coleoptera. Of these 19 new species, seven produce a sexual morph (M. clavatum, M. eburneum, M. flavum, M. phuwiangense, M. purpureonigrum, M. purpureum, and M. sulphureum) and asexual morphs are found in the remaining new species and also in M. sulphureum, M. purpureonigrum and M. purpureum. Metarhizium blattodeae, M. koreanum and M. viridulum are new records for Thailand. An alternative neotype for Metarhizium anisopliae is proposed based on multi-gene and 50 tef analyses showing that CBS 130.71 from Ukraine is more suitable, being from a much closer geographical location to Metchnikoff's Metarhizium anisopliae. This isolate is distinct from the neotype of Metarhizium anisopliae var. anisopliae proposed by M. Tulloch from Ethiopia (ARSEF 7487). Six new genera are established for monophyletic clades subtending the core Metarhizium clade, including Keithomyces, Marquandomyces, Papiliomyces, Purpureomyces, Sungia, and Yosiokobayasia. Metarhizium carneum, M. aciculare, and M. neogunnii are combined in Keithomyces and one new combination for M. marquandii in Marquandomyces is proposed. Purpureomyces is introduced for species producing purple stromata including a new combination for M. khaoyaiense and two new species P. maesotensis and P. pyriformis. Papiliomyces contains two new combinations for M. liangshanense and Metacordyceps shibinensis. The genus Sungia is proposed for the Korean species M. yongmunense on Lepidoptera pupa and Yosiokobayasia for the Japanese species M. kusanagiense also on Lepidoptera pupa. A synoptic and dichotomous key to the accepted taxa is provided together with tables listing distinguishing morphological characters between species, host preferences, and geography.

引用

页码：171 / 251

页数：81

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