Tracking Multiplex Drugs and Their Dynamics in Living Cells Using the Label-Free Surface-Enhanced Raman Scattering Technique

被引：31
作者：
Yang, Jing [1 ]
Cui, Yiping [1 ]
Zong, Shenfei [1 ]
Zhang, Ruohu [1 ]
Song, Chunyuan [1 ]
Wang, Zhuyuan [1 ]
机构：
[1] Southeast Univ, Adv Photon Ctr, Nanjing 210096, Jiangsu, Peoples R China
来源：
MOLECULAR PHARMACEUTICS | 2012年 / 9卷 / 04期
基金：
美国国家科学基金会;
关键词：
SERS; silver nanoparticles; drugs; metabolism; cells; THIOPURINE S-METHYLTRANSFERASE; DENSITY-FUNCTIONAL THEORY; IN-SITU SERS; LYMPHOBLASTIC-LEUKEMIA; 6-MERCAPTOPURINE SAMS; SILVER ELECTRODE; NANOPARTICLES; SPECTROSCOPY; METHIMAZOLE; ADSORPTION;
D O I：
10.1021/mp200667d
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Label free and real time detection of nonfluorescent drugs inside living cells has been realized by using surface-enhanced Raman scattering (SERS). For the first time, the characteristics of 6-mercapotopurine and methimazole, two different drugs, were monitored simultaneously by SERS in living cells. Particularly, the processes of diffusion and metabolism of drugs occurring in the intracellular matrix were investigated. The results indicate that the metabolism speed of 6-mercapotopurine in living He La cells is much faster than that of methimazole. Moreover, the detection sensitivity of intracellular drugs has also been checked and a low detection limit of 1 nM was obtained of drug 6-mercapotopurine in a single HeLa cell.
引用
收藏
页码：842 / 849
页数：8
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