Molecular differential expression of voltage-gated sodium channel α and β subunit mRNAs in five different mammalian cell lines

被引:17
作者
Baroni, Debora [1 ]
Moran, Oscar [1 ]
机构
[1] CNR, Ist Biofis, I-16149 Genoa, Italy
关键词
Voltage-gated sodium channel; alpha subunit; beta subunits; mRNA expression; INHERITED CARDIAC-ARRHYTHMIA; CENTRAL-NERVOUS-SYSTEM; FEBRILE SEIZURES PLUS; HUMAN SKELETAL-MUSCLE; DORSAL-ROOT GANGLIA; LONG-QT SYNDROME; NA+-CHANNEL; FUNCTIONAL EXPRESSION; RAT-BRAIN; GENERALIZED EPILEPSY;
D O I
10.1007/s10863-011-9399-7
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Voltage-gated sodium channels are composed of one alpha subunit and one or more auxiliary beta subunits. A standard reverse transcription-polymerase chain reaction assay was used to detect the mRNAs encoding for seven alpha subunits (Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.5, Nav1.6, Nav1.9) and for the two non-covalently linked beta 1 and beta 3 auxiliary subunits in five different cell lines from rat, mouse and human origin. A semi-quantitavive RT-PCR analysis allowed to evaluate in each cell line, the relative expression level of each NaCh subunit previously detected. The expression profile of the cell lines was compared with that obtained from rat and mouse neural, skeletal muscle and cardiac tissues. This data provide a standard for the study of the modulation of the sodium channel expression in mammalian excitable tissues.
引用
收藏
页码:729 / 738
页数:10
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