Genetic relationship among 19 accessions of six species of Chenopodium L., by Random Amplified Polymorphic DNA fragments (RAPD)

被引：48
作者：
Ruas, PM [1 ]
Bonifacio, A
Ruas, CF
Fairbanks, DJ
Andersen, WR
机构：
[1] Univ Estadual Londrina, Dept Biol Geral, BR-86051990 Londrina, Parana, Brazil
[2] Bolivian Inst Agr Technol, La Paz, Bolivia
[3] Brigham Young Univ, Dept Bot & Range Sci, Provo, UT USA
来源：
EUPHYTICA | 1999年 / 105卷 / 01期
关键词：
Chenopodium; genetic relationship; molecular markers; RAPD;
D O I：
10.1023/A:1003480414735
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The RAPD technique was used to identify genetic relationships in 19 accessions, including six species of the genus Chenopodium. A dendrogram was constructed using UPGMA from 399 DNA markers. The molecular data clustered species and accessions into five different groups. Group 1 with three cultivated varieties of C. nuttalliae, Group 2 included eight cultivars and two wild varieties of C. quinoa, Group 3 with C. berlandieri and C. album, Group 4 with two accessions of C, pallidicaule, and Group 5 with 2 accessions of C. ambrosioides. The polymorphic patterns generated by RAPD profiles showed different degrees of genetic relationship among the species studied. A low level of intraspecific variation was found within the accessions of C. quinoa, C. nuttalliae, and C. pallidicaule. The RAPD markers were found to be a useful tool for detecting genetic variation within the genus Chenopodium.
引用
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页码：25 / 32
页数：8
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