Effective and Novel Application of Hydrodynamic Voltammetry to the Study of Superoxide Radical Scavenging by Natural Phenolic Antioxidants

被引：33

作者：

Belli, Stuart ^{[1
]}

Rossi, Miriam ^{[1
]}

Molasky, Nora ^{[1
]}

Middleton, Lauren ^{[1
]}

Caldwell, Charles ^{[1
]}

Bartow-McKenney, Casey ^{[1
]}

Duong, Michelle ^{[1
]}

Chiu, Jana ^{[1
]}

Gibbs, Elizabeth ^{[1
]}

Caldwell, Allison ^{[1
]}

Gahn, Christopher ^{[2
,3
]}

Caruso, Francesco ^{[1
]}

机构：

[1] Vassar Coll, Dept Chem, Poughkeepsie, NY 12604 USA

[2] Vassar Coll, Comp Serv, Poughkeepsie, NY 12604 USA

[3] Vassar Coll, Informat Serv, Poughkeepsie, NY 12604 USA

来源：

ANTIOXIDANTS | 2019年 / 8卷 / 01期

基金：

美国国家科学基金会;

关键词：

antioxidants; flavonoid; superoxide; cyclic voltammetry; ROS; APPROXIMATION; FLAVONOIDS; MOLECULES; OXIDATION; CAPACITY; SURFACES; EXCHANGE; SOLIDS;

D O I：

10.3390/antiox8010014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The reactions of antioxidants with superoxide radical were studied by cyclic voltammetry (CV)-and hydrodynamic voltammetry at a rotating ring-disk electrode (RRDE). In both methods, the superoxide is generated in solution from dissolved oxygen and then measured after being allowed to react with the antioxidant being studied. Both methods detected and measured the radical scavenging but the RRDE was able to give detailed insight into the antioxidant behavior. Three flavonoids, chrysin, quercetin and eriodictyol, were studied, their scavenging activity of superoxide was assessed and the molecular structure of each flavonoid was related to its scavenging capability. From our improved and novel RRDE method, we determine the ability of these 3 antioxidants to react with superoxide radical in a more quantitative manner than the classical CV. Density Functional Theory (DFT) and single crystal X-ray diffraction data provide structural information that assists in clarifying the scavenging molecular mechanism. Hydroxyls associated with the A ring, as found in chrysin, scavenge superoxide in a different manner than those found in the B ring of flavonoids, as those in quercetin and eriodictyol.

引用

页数：20

共 31 条

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