Glutathione S-transferase influences the fecundity of Schistosoma japonicum

被引：20

作者：

Tang, Chun-lian ^{[1
]}

Zhou, Hong-hua ^{[1
]}

Zhu, Ya-wen ^{[1
]}

Huang, Jin ^{[2
]}

Wang, Guo-bo ^{[1
]}

机构：

[1] Wuhan Univ Sci & Technol, Wuchang Hosp, Wuhan 430063, Hubei, Peoples R China

[2] Huazhong Univ Sci & Technol, Dept Clin Lab, Wuhan Hosp 4, Puai Hosp,Tongji Med Coll, Wuhan 430033, Hubei, Peoples R China

来源：

ACTA TROPICA | 2019年 / 191卷

关键词：

Schistosoma japonicum; Glutathione S-transferase; Egg; RNA interference; MANSONI; VACCINE; CLONING;

D O I：

10.1016/j.actatropica.2018.12.027

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The aim of this study was to investigate the effect of Schistosoma japonicwn glutathione S-transferase (SjGST) on the developmental stages of the parasite. We found that the mRNA levels of GST were higher in schistosomula obtained from the host and the eggs than that in other developmental stages. SjGST was mainly distributed in the egg shells, teguments of the worms, and part of the parenchyma of the worms. GST knockdown with RNA interference in S. japonicum worms resulted in a silencing rate higher than 80%. The egg reduction rate (18%) and abnormal egg ratio (28%) were significantly higher (P < 0.05) in the GST-silenced group than in the negative control group. These results indicate that SjGST plays an important role in the fecundity of S. japonicum, specifically in egg formation.

引用

页码：8 / 12

页数：5

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