Infliximab protects against pulmonary emphysema in smoking rats

被引：16

作者：

Zhang Xiang-yan ^{[1
]}

Zhang Cheng ^{[1
]}

Sun Qian-yun ^{[2
,3
]}

Li Dan ^{[1
]}

Luo Rong-rong ^{[1
]}

Wan Zi-fen ^{[1
]}

Ye Xian-wei ^{[1
]}

Liu Wei-jia ^{[1
]}

Rao Shan-shan ^{[1
]}

Jing, Han ^{[1
]}

机构：

[1] Guizhou Prov Peoples Hosp, Dept Resp Med, Guiyang 550002, Guizhou, Peoples R China

[2] Key Lab Chem Nat Prod Guizhou Prov, Guiyang 550002, Guizhou, Peoples R China

[3] Chinese Acad Sci, Guiyang 550002, Guizhou, Peoples R China

来源：

CHINESE MEDICAL JOURNAL | 2011年 / 124卷 / 16期

关键词：

tumor necrosis factor-alpha; infliximab; emphysema; rat; N-ACETYLCYSTEINE; CELL APOPTOSIS; DISEASE; VEGF;

D O I：

10.3760/cma.j.issn.0366-6999.2011.16.019

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background It is widely accepted that tumor necrosis factor-alpha (TNF-alpha) plays an important role in the pathogenesis of emphysema. This study aimed at investigating the protective effects of anti-TNF-alpha antibody, infliximab, in the development of emphysema induced by passive smoking in rats. Methods Thirty-nine rats were randomly divided into a normal control group (group 1), an emphysema group (group 2), and an infliximab-intervention group (group 3). Rat models of emphysema were established by exposure to cigarette smoking daily for 74 days. After 1 month, the infliximab intervention group was treated with infliximab via subcutaneous injection. The levels of TNF-alpha, IL-8 and vascular endothelial growth factor (VEGF) in bronchoalveolar lavage fluid (BALF) were measured with enzyme linked immunosorbent assay (ELISA). The number and classification of cells in the BALF were measured. Lung tissue sections stained by hematoxylin and eosin (HE) were observed, and mean linear intercept (MLI) and mean alveolar numbers (MAN) were measured. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) methods were used to examine the percentage of positive cells and distribution of apoptotic cells. Results The levels of TNF-alpha and IL-8 in BALF were higher in group 2 than in group 1 and group 3. The MLI was greater in group 2 than that in group 1 and group 3 while MAN was decreased. The concentration of VEGF in BALF of group 2 was significantly decreased as compared with group 1. The total cells and neutrophils number was significantly increased in group 2 as compared with group 1 and group 3, so was the percentage of neutrophils. The number of TUNEL positive cells in the alveolar septa was significantly increased in group 2 as compared with group 1 and group 3. Conclusion Infliximab protects against cigarette smoking-induced emphysema by reducing airway inflammation, attenuating alveolar septa cell apoptosis and improving pathological changes. Chin Med J 2011;124(16):2502-2506

引用

页码：2502 / 2506

页数：5

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