The prevalence of Chlamydia trachomatis and Neisseria gonorrhoeae in the endocervical swab specimens of symptomatic, asymptomatic and infertile women in Turkey

被引:12
作者
Eksi, Fahriye [1 ]
Dikensoy, Ebru [2 ]
Gayyurhan, Efgan Dogan [1 ]
Balci, Iclal [1 ]
Balat, Ozcan [2 ]
Karsligil, Tekin [1 ]
Bayram, Aysen [1 ]
Komurcu, Ozge [2 ]
机构
[1] Gaziantep Univ, Dept Med Microbiol, Fac Med, TR-27310 Gaziantep, Turkey
[2] Gaziantep Univ, Dept Obstet & Gynecol, Fac Med, TR-27310 Gaziantep, Turkey
关键词
C; trachomatis; N; gonorrhoeae; Symptomatic women; Asymptomatic women; Infertile women; STRAND DISPLACEMENT AMPLIFICATION; ABBOTT LCX ASSAY; INFECTIONS; CULTURE; DIAGNOSIS; SYSTEM;
D O I
10.1007/s00404-010-1694-8
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
To investigate C. trachomatis and N. gonorrhoeae prevalence in three different female populations in Turkey. A total of 370 women, 170 symptomatic, 100 asymptomatic, and 100 infertile, were included. Of the endocervical specimens collected from all women using a Dacron swab, the first one was taken to Stuart's transport medium to culture, while the second one was transferred onto slides to perform direct fluorescent antibody test (DFA) and Gram staining, and the third specimen was used for Becton Dickinson BDProbeTec ET system (BDPT). C. trachomatis was detected in 5.16% of symptomatic, 1.11% of asymptomatic, and 2.15% of infertile women with BDPT. Sensitivity and specificity of the DFA test were 72.73 and 97.85%, respectively. N. gonorrhoeae was detected in 2.42% of symptomatic and in 1.02% of infertile women. N. gonorrhoeae was not detected in any asymptomatic women. In N. gonorrhoeae-positive patients, sensitivity and specificity of culture were 60 and 100%, respectively, while they were 80 and 100% for BDPT. Prevalence of N. gonorrhoeae and C. trachomatis was detected to be low in Turkish women, and the difference between the groups was not significant. Both agents were more prevalent in subjects over 25 years of age.
引用
收藏
页码:1349 / 1355
页数:7
相关论文
共 30 条
[1]  
Açar A, 2008, TURK J OBSTET GYNECO, V5, P57
[2]  
Acik Y, 2004, ERCIYES MED J, V26, P55
[3]  
[Anonymous], 1995, Sex Transm Dis, V22, P71
[4]   Comparison of the APTIMA CT and GC assays with the APTIMA combo 2 assay, the Abbott LCx assay, and direct fluorescent-antibody and culture assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae [J].
Boyadzhyan, B ;
Yashina, T ;
Yatabe, JH ;
Patnaik, M ;
Hill, CS .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (07) :3089-3093
[5]   Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by ligase chain reaction-based assays with clinical specimens from various sites: Implications for diagnostic testing and screening [J].
Buimer, M ;
VanDoornum, GJJ ;
Ching, S ;
Peerbooms, PGH ;
Plier, PK ;
Ram, D ;
Lee, HH .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (10) :2395-2400
[6]   Neisseria gonorrhoeae contains multiple copies of a gene that may encode a site-specific recombinase and is associated with DNA rearrangements [J].
Carrick, CS ;
Fyfe, JAM ;
Davies, JK .
GENE, 1998, 220 (1-2) :21-29
[7]   High analytical sensitivity and low rates of inhibition may contribute to detection of Chlamydia trachomatis in significantly more women by the APTIMA Combo 2 assay [J].
Chernesky, M ;
Jang, D ;
Luinstra, K ;
Chong, S ;
Smieja, M ;
Cai, WJ ;
Hayhoe, B ;
Portillo, E ;
MacRitchie, C ;
Main, C ;
Ewert, R .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (02) :400-405
[8]   Assessment of Chlamydia trachomatis prevalence by cell culture and transcription-mediated amplification in symptomatic women [J].
Cicek, C ;
Altuglu, I ;
Ozacar, T ;
Kolday, K ;
Demir, N ;
Bilgic, A .
MEDICAL PRINCIPLES AND PRACTICE, 2004, 13 (02) :91-94
[9]   Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by strand displacement amplification and relevance of the amplification control for use with vaginal swab specimens [J].
Cosentino, LA ;
Landers, DV ;
Hillier, SL .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (08) :3592-3596
[10]   Detection of Neisseria gonorrhoeae and Chlamydia trachomatis in genitourinary specimens from men and women by a coamplification PCR assay [J].
Crotchfelt, KA ;
Welsh, LE ;
DeBonville, D ;
Rosenstraus, M ;
Quinn, TC .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (06) :1536-1540