Large-Conductance Transmembrane Porin Made from DNA Origami

被引：152

作者：

Gopfrich, Kerstin ^{[1
]}

Li, Chen-Yu ^{[2
]}

Ricci, Maria ^{[1
]}

Bhamidimarri, Satya Prathyusha ^{[3
]}

Yoo, Jejoong ^{[2
]}

Gyenes, Bertalan ^{[1
]}

Ohmann, Alexander ^{[1
]}

Winterhalter, Mathias ^{[3
]}

Aksimentiev, Aleksei ^{[2
]}

Keyser, Ulrich F. ^{[1
]}

机构：

[1] Univ Cambridge, Cavendish Lab, Cambridge CB3 0HE, England

[2] Univ Illinois, Dept Phys, Ctr Phys Living Cells, 1110 West Green St, Urbana, IL 61801 USA

[3] Jacobs Univ Bremen, D-28759 Bremen, Germany

来源：

ACS NANO | 2016年 / 10卷 / 09期

基金：

美国国家科学基金会; 瑞士国家科学基金会;

关键词：

DNA origami; lipid membrane; synthetic porin; ionic current recordings; molecular dynamics; MOLECULAR-DYNAMICS; MEMBRANE; NANOPORES; CHANNELS; TRANSLOCATION; SIMULATION; ALGORITHM; TRANSPORT; VERSION; SHAPES;

D O I：

10.1021/acsnano.6b03759

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

DNA nanotechnology allows for the creation of three-dimensional structures at nanometer scale. Here, we use DNA to build the largest synthetic pore in a lipid membrane: to date, approaching the dimensions of the nuclear pore complex and increasing the pore-area and the conductance 10-fold compared to previous man-made channels. In our design, 19 cholesterol tags anchor a megadalton funnel-shaped DNA origami porin in a lipid bilayer membrane. Confocal imaging and ionic current recordings reveal spontaneous insertion of the. DNA porin into the lipid membrane, creating a transmethbrane pore of tens of nanosiemens conductance. All-atom molecular dynamics simulations characterize the conductance mechanism at the atomic level and independently confirm the DNA porins' large ionic conductance.

引用

页码：8207 / 8214

页数：8

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