Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework

被引：130

作者：

Song, Ping ^{[1
,2
]}

Shen, Juwen ^{[3
]}

Ye, Dekai ^{[4
]}

Dong, Baijun ^{[1
]}

Wang, Fei ^{[2
]}

Pei, Hao ^{[5
]}

Wang, Jianbang ^{[2
]}

Shi, Jiye ^{[4
]}

Wang, Lihua ^{[4
,6
]}

Xue, Wei ^{[1
]}

Huang, Yiran ^{[1
]}

Huang, Gang ^{[1
]}

Zuo, Xiaolei ^{[1
,2
]}

Fan, Chunhai ^{[1
,2
]}

机构：

[1] Shanghai Jiao Tong Univ, Inst Mol Med, Dept Urol, Dept Nucl Med,Renji Hosp,Sch Med, Shanghai 200127, Peoples R China

[2] Shanghai Jiao Tong Univ, Frontiers Sci Ctr Transformat Mol, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China

[3] East China Normal Univ, Shanghai Key Lab Regulatory Biol, Inst Biomed Sci, Sch Life Sci, Shanghai 200241, Peoples R China

[4] Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Div Phys Biol, Shanghai 201800, Peoples R China

[5] East China Normal Univ, Shanghai Key Lab Green Chem & Chem Proc, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China

[6] Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Bioimaging Ctr,Shanghai Synchrotron Radiat Facil, Shanghai 201210, Peoples R China

来源：

NATURE COMMUNICATIONS | 2020年 / 11卷 / 01期

关键词：

GLUCOSE-RESPONSIVE INSULIN; PROSTATE-CANCER; SARCOSINE; CELLS; NANOSTRUCTURES; METABOLISM; LIPOSOMES; MECHANISM; BIOMARKER; MACHINES;

D O I：

10.1038/s41467-020-14664-8

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Protein-protein interactions are spatially regulated in living cells to realize high reaction efficiency, as seen in naturally existing electron-transfer chains. Nevertheless, arrangement of chemical/biochemical components at the artificial device interfaces does not possess the same level of control. Here we report a tetrahedral DNA framework-enabled bulk enzyme heterojunction (BEH) strategy to program the multi-enzyme catalytic cascade at the interface of electrochemical biosensors. The construction of interpenetrating network of BEH at the millimeter-scale electrode interface brings enzyme pairs within the critical coupling length (CCL) of similar to 10nm, which in turn greatly improve the overall catalytic cascade efficiency by similar to 10-fold. We demonstrate the BEH generality with a range of enzyme pairs for electrochemically detecting clinically relevant molecular targets. As a proof of concept, a BEH-based sarcosine sensor enables single-step detection of the metabolic biomarker of sarcosine with ultrasensitivity, which hold the potential for precision diagnosis of early-stage prostate cancer.

引用

页数：10

共 49 条

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