Evaluation of an Ultrafiltration-Based Procedure for Simultaneous Recovery of Diverse Microbes in Source Waters

被引：32

作者：

Kahler, Amy M. ^{[1
]}

Johnson, Trisha B. ^{[2
]}

Hahn, Donghyun ^{[2
]}

Narayanan, Jothikumar ^{[1
]}

Derado, Gordana ^{[1
]}

Hill, Vincent R. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30329 USA

[2] Atlanta Res & Educ Fdn, Decatur, GA 30033 USA

来源：

WATER | 2015年 / 7卷 / 03期

关键词：

HOLLOW-FIBER ULTRAFILTRATION; CRYPTOSPORIDIUM-PARVUM OOCYSTS; DEAD-END ULTRAFILTRATION; SURFACE-WATER; IMMUNOMAGNETIC SEPARATION; DRINKING-WATER; SAMPLES; VIRUSES; ENTEROVIRUSES; FILTER;

D O I：

10.3390/w7031202

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In this study, hollow-fiber ultrafiltration (UF) was assessed for recovery of Escherichia coli, Clostridium perfringens spores, Cryptosporidium parvum oocysts, echovirus 1, and bacteriophages MS2 and phi X174 from ground and surface waters. Microbes were seeded into twenty-two 50-L water samples that were collected from the Southeastern United States and concentrated to ~500 mL by UF. Secondary concentration was performed for C. parvum by centrifugation followed by immunomagnetic separation. Secondary concentration for viruses was performed using centrifugal ultrafilters or polyethylene glycol precipitation. Nine water quality parameters were measured in each water sample to determine whether water quality data correlated with UF and secondary concentration recovery efficiencies. Average UF recovery efficiencies were 66%-95% for the six enteric microbes. Average recovery efficiencies for the secondary concentration methods were 35%-95% for C. parvum and the viruses. Overall, measured water quality parameters were not significantly associated with UF recovery efficiencies. However, recovery of phi X174 was negatively correlated with turbidity. The recovery data demonstrate that UF can be an effective method for concentrating diverse microbes from ground and surface waters. This study highlights the utility of tangential-flow hollow fiber ultrafiltration for recovery of bacteria, viruses, and parasites from large volume environmental water samples.

引用

页码：1202 / 1216

页数：15

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