Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells

被引：0

作者：

Yamashita, Makiko

Katakura, Yoshinori

Aiba, Yoshihiro

Matsumoto, Shin-ei

Morihara, Kazuko

Teruya, Kiichiro

Ichikawa, Akira

Shirahata, Sanetaka

机构：

[1] Kyushu Univ, Grad Sch Syst Life Sci, Higashi Ku, Fukuoka 8128581, Japan

[2] Kyushu Univ, Fac Agr, Dept Genet Resources Technol, Higashi Ku, Fukuoka 8128581, Japan

[3] Kyoto Inst Technol, Dept Appl Biol, Sakyo Ku, Kyoto 6068585, Japan

来源：

CYTOTECHNOLOGY | 2007年 / 55卷 / 2-3期

关键词：

Antibody production; Interleukin-10; CD11c(+) dendritic cells; In vitro immunization; Peripheral blood mononuclear cells;

D O I：

10.1007/s10616-007-9088-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Previously, we have established an in vitro immunization method to induce antigen-specific antibody-producing B cells. In the present study, we have attempted to clarify the mechanisms that regulate antibody production by in vitro immunized peripheral blood mononuclear cells (PBMC). Freshly isolated PBMC did not induce antibody production following in vitro immunization, but expressed the interleukin (IL)-10 gene. On the other hand, PBMC pretreated with L-leucyl-L-leucine (LLME) induced antibody production, but did not express the IL-10 gene. IL-10 induced functional impairment of CD4+ Th cells and CD11c(+) DC, resulting in the suppression of antibody production by in vitro immunized PBMC.

引用

页码：71 / 77

页数：7

共 18 条

[11] Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25+CD4+ regulatory cells that control intestinal inflammation [J].