Guidelines for the measurement of BCR-ABL1 transcripts in chronic myeloid leukaemia

被引：86

作者：

Foroni, Letizia ^{[1
]}

Wilson, Gill ^{[2
]}

Gerrard, Gareth ^{[1
]}

Mason, Joanne ^{[3
]}

Grimwade, David ^{[4
]}

White, Helen E. ^{[5
]}

de Castro, David Gonzalez ^{[6
]}

Austin, Stephen ^{[7
]}

Awan, Abida ^{[8
]}

Burt, Emma ^{[9
]}

Clench, Tim ^{[10
]}

Farruggia, Joanna ^{[11
]}

Hancock, Jeremy ^{[12
]}

Irvine, Alexandra E. ^{[13
]}

Kizilors, Aytug ^{[14
]}

Langabeer, Stephen ^{[15
]}

Milner, Benedict J. ^{[16
]}

Nickless, Guillermina ^{[17
]}

Schuh, Anna ^{[18
]}

Sproul, Anne ^{[19
]}

Wang, Lihui ^{[20
]}

Wickham, Caroline ^{[21
,22
]}

Cross, Nicholas C. P. ^{[5
,23
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Acad Hlth Sci Ctr, London SW7 2AZ, England

[2] Sheffield Childrens NHS Fdn Trust, Sheffield, S Yorkshire, England

[3] Birmingham Womens Hosp, W Midlands Reg Genet Lab, Birmingham, W Midlands, England

[4] Kings Coll London, Sch Med, London, England

[5] Natl Genet Reference Lab Wessex, Salisbury, Wilts, England

[6] Royal Marsden NHS Fdn Trust, Sutton, Surrey, England

[7] Cardiff & Vale Univ Hlth Board, Cardiff, S Glam, Wales

[8] Manchester Royal Infirm, Manchester, Lancs, England

[9] Barts & London NHS Trust, London, England

[10] Bristol Royal Infirm & Gen Hosp, Bristol, Avon, England

[11] Derriford Hosp, Plymouth PL6 8DH, Devon, England

[12] Southmead Hosp, Bristol Genet Lab, Bristol, Avon, England

[13] Ctr Canc Res & Cell Biol, Belfast, Antrim, North Ireland

[14] Kings Coll Hosp London, Lab Mol Haematooncol, London, England

[15] St James Hosp, Dublin, Ireland

[16] Aberdeen Royal Infirm, Aberdeen, Scotland

[17] Guys Hosp, London, England

[18] John Radcliffe Hosp, Oxford, England

[19] Western Gen Hosp, Edinburgh EH4 2XU, Midlothian, Scotland

[20] Royal Liverpool Univ Hosp, Liverpool, Merseyside, England

[21] Royal Devon & Exeter NHS Fdn Trust, Exeter, Devon, England

[22] Peninsula Coll Med & Dent, Plymouth, Devon, England

[23] Univ Southampton, Sch Med, Southampton SO9 5NH, Hants, England

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 2011年 / 153卷 / 02期

关键词：

BCR; ABL1; real time PCR; guidelines; chronic myeloid leukaemia; POLYMERASE-CHAIN-REACTION; BCR-ABL TRANSCRIPTS; HARMONIZING CURRENT METHODOLOGY; RESIDUAL DISEASE DETECTION; FUSION GENE TRANSCRIPTS; TYROSINE KINASE; IMATINIB; CANCER; DIAGNOSIS; PATIENT;

D O I：

10.1111/j.1365-2141.2011.08603.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

P>Molecular testing for the BCR-ABL1 fusion gene by real time quantitative polymerase chain reaction (RT-qPCR) is the most sensitive routine approach for monitoring the response to therapy of patients with chronic myeloid leukaemia. In the context of tyrosine kinase inhibitor (TKI) therapy, the technique is most appropriate for patients who have achieved complete cytogenetic remission and can be used to define specific therapeutic milestones. To achieve this effectively, standardization of the laboratory procedures and the interpretation of results are essential. We present here consensus best practice guidelines for RT-qPCR testing, data interpretation and reporting that have been drawn up and agreed by a consortium of 21 testing laboratories in the United Kingdom and Ireland in accordance with the procedures of the UK Clinical Molecular Genetics Society.

引用

页码：179 / 190

页数：12

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