Cytokeratin 13, Cytokeratin 17, and Ki-67 Expression in Human Acquired Cholesteatoma and Their Correlation With Its Destructive Capacity

被引：18

作者：

Hamed, Mahmood A. ^{[1
,2
]}

Nakata, Seiichi ^{[1
]}

Shiogama, Kazuya ^{[3
]}

Suzuki, Kenji ^{[1
,4
]}

Sayed, Ramadah H. ^{[2
]}

Nishimura, Yoichi ^{[1
]}

Iwata, Noboru ^{[1
]}

Sakurai, Kouhei ^{[5
]}

Bedawy, Badawy S. ^{[2
]}

Inada, Ken-ichi ^{[5
]}

Tsuge, Hayato ^{[6
]}

Tsutsumi, Yutaka ^{[3
]}

机构：

[1] Fujita Hlth Univ, Banbuntane Hotokukai Hosp, Dept Otorhinolaryngol, Sch Med, Nagoya, Aichi, Japan

[2] Sohag Univ, Dept Otorhinolaryngol, Sohag Fac Med, Sohag, Egypt

[3] Fujita Hlth Univ, Dept Pathol, Sch Med, Toyoake, Aichi, Japan

[4] Yonaha Gen Hosp, Dept Otorhinolaryngol, Kuwana, Japan

[5] Fujita Hlth Univ, Banbuntane Hotokukai Hosp, Dept Pathol, Sch Med, Nagoya, Aichi, Japan

[6] Red Cross Nagoya Daiichi Hosp, Dept Otorhinolaryngol, Nagoya, Aichi, Japan

来源：

CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY | 2017年 / 10卷 / 03期

关键词：

Cholesteatoma; Middle Ear; Bone Resorption; Ki-67; Antigen; Cytokeratin; 17; MIDDLE-EAR CHOLESTEATOMA; CHRONIC OTITIS-MEDIA; BONE DESTRUCTION; GROWTH-FACTOR; EPITHELIAL PROLIFERATION; TELOMERASE ACTIVITY; FACTOR-ALPHA; PATTERNS; ETIOPATHOGENESIS; DIFFERENTIATION;

D O I：

10.21053/ceo.2016.01263

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

Objectives. Cholesteatoma is a nonneoplastic destructive lesion of the temporal bone with debated pathogenesis and bone resorptive mechanism. Both molecular and cellular events chiefly master its activity. Continued research is necessary to clarify factors related to its aggressiveness. We aimed to investigate the expression of Ki-67, cytokeratin 13 (CK13) and cytokeratin 17 (CK17) in acquired nonrecurrent human cholesteatoma and correlate them with its bone destructive capacity. Methods. A prospective quantitative immunohistochemical study was carried out using fresh acquired cholesteatoma tissues (n=19), collected during cholesteatoma surgery. Deep meatal skin tissues from the same patients were used as control (n=8). Cholesteatoma patients were divided into 2 groups and compared (invasive and noninvasive) according to a grading score for bone resorption based upon clinical, radiologic and intraoperative findings. To our knowledge, the role' of CK17 in cholesteatoma aggressiveness was first investigated in this paper. Results. Both Ki-67 and CK17 were significantly overexpressed in cholesteatoma than control tissues (P <0.001 for both Ki-67 and CK17). In addition, Ki-67 and CK17 were significantly higher in the invasive group than noninvasive group of cholesteatoma (P=0.029, P=0.033, respectively). Furthermore, ki-67 and CK17 showed a moderate positive correlation with bone erosion scores (r= 0.547, P=0.015 and r=0.588, P= 0.008, respectively). In terms of CK13, no significant difference was found between cholesteatoma and skin (P=0.766). Conclusion. Both Ki-67 and CK17 were overexpressed in cholesteatoma tissue and positively correlated with bone resorption activity. The concept that Ki-67 can be a predictor for aggressiveness of cholesteatoma was supported. In addition, this is the first study demonstrating CK17 as a favoring marker in the aggressiveness of acquired cholesteatoma.

引用

页码：213 / 220

页数：8

共 30 条

[11] Expression patterns of Ki-67 and telomerase activity in middle ear cholesteatoma [J].