Supplementation of lycopene in maturation media improves bovine embryo quality in vitro

被引：30

作者：

Chowdhury, M. M. R. ^{[1
,4
]}

Choi, Byung-Hyun ^{[1
]}

Khan, Imran ^{[1
]}

Lee, Kyeong-Lim ^{[1
]}

Mesalam, Ayman ^{[1
]}

Song, Seok-Hwan ^{[1
]}

Xu, Lianguang ^{[1
]}

Joo, Myeong-Don ^{[1
]}

Afrin, Fahmida ^{[3
]}

Kong, Il-Keun ^{[1
,2
]}

机构：

[1] Gyeongsang Natl Univ, Div Appl Life Sci Plus BK21, Dept Anim Sci, Jinju 660701, Gyeongnam Provi, South Korea

[2] Gyeongsang Natl Univ, Inst Agr & Life Sci, Jinju 660701, Gyeongnam Provi, South Korea

[3] Gyeongsang Natl Univ, Dept Microbiol & Virol, Coll Vet Med, Jinju 52828, Gyeongnam Provi, South Korea

[4] Patuakhali Sci & Technol Univ, Fac Anim Sci & Vet Med, Dept Physiol & Pharmacol, Patuakhali 8210, Bangladesh

来源：

THERIOGENOLOGY | 2017年 / 103卷

关键词：

Lycopene; Oxidative stress; Free radicals; Bovine; Embryo culture; NF-KAPPA-B; OXIDATIVE STRESS; FREE-RADICALS; DEVELOPMENTAL COMPETENCE; DNA-DAMAGE; VITAMIN-E; CULTURE; OOCYTES; CAROTENOIDS; ANTIOXIDANT;

D O I：

10.1016/j.theriogenology.2017.08.003

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This study sought to modulate factors that reduce embryo quality in in vitro culture (IVC) systems. Over eight replicates, 3075 oocytes were cultured in in vitro maturation media containing various concentrations of lycopene, followed by in vitro fertilization and culture. The percentages of MII-stage oocytes, the presumptive zygotes that underwent cleavage and developed into blastocysts were significantly (P < 0.05) higher, the intracellular ROS concentrations reduced significantly (P < 0.05) in oocytes/blastocysts, TUNEL assay demonstrates reduced apoptosis and increased total cell number per blastocyst (P < 0.05), Immunocytochemistry confirmed that diminished protein expression of nuclear factor kappa B (NF kappa B), cyclooxygenase-2 (COX2), and 8-oxoguanine (indicated by ROS) and relative mRNA expression of the Caspase-3, NF kappa B, COX2, iNOS and BCL2-associated X (BAX) was significantly (P < 0.05) lower whereas the anti-apoptotic gene BCL2 was significantly (P < 0.05) higher in the 0.2 mu M lycopene-supplemented group than the control. In conclusion, lycopene improves blastocyst quality by overcoming unfavorable conditions in in vitro culture systems. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：173 / 184

页数：12

共 73 条

[1] Accumulation of cytoplasmic lipid droplets in bovine embryos and cryotolerance of embryos developed in different culture systems using serum-free or serum-containing media [J].