The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae

被引:12
作者
Raya Tonetti, Fernanda [1 ]
Tomokiyo, Mikado [2 ,3 ]
Ortiz Moyano, Ramiro [1 ]
Quilodran-Vega, Sandra [4 ]
Yamamuro, Hikari [2 ,3 ]
Kanmani, Paulraj [2 ]
Melnikov, Vyacheslav [5 ]
Kurata, Shoichiro [6 ]
Kitazawa, Haruki [2 ,3 ]
Villena, Julio [1 ,2 ]
机构
[1] Reference Ctr Lactobacilli CERELA CONICET, Lab Immunobiotechnol, RA-4000 San Miguel De Tucuman, Argentina
[2] Tohoku Univ, Grad Sch Agr Sci, Lab Anim Food Funct, Food & Feed Immunol Grp, Sendai, Miyagi 9808572, Japan
[3] Tohoku Univ, Int Educ & Res Ctr Food & Agr Immunol CFAI, Grad Sch Agr Sci, Livestock Immunol Unit, Sendai, Miyagi 9808572, Japan
[4] Univ Concepcion, Fac Vet Sci, Lab Food Microbiol, Chillan 3780000, Chile
[5] Gabrichevsky Res Inst Epidemiol & Microbiol, Moscow 125212, Russia
[6] Tohoku Univ, Grad Sch Pharmaceut Sci, Lab Mol Genet, Sendai, Miyagi 9808578, Japan
基金
日本学术振兴会;
关键词
respiratory commensal bacteria; upper respiratory tract; next-generation probiotics; Dolosigranulum pigrum; Streptococcus pneumoniae; alveolar macrophages; MICROBIAL COMMUNITIES; TRACT INFECTIONS; NEUTROPHILS; RESISTANCE; HEALTHY;
D O I
10.3390/microorganisms9061324
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Previously, we demonstrated that the nasal administration of Dolosigranulum pigrum 040417 differentially modulated the respiratory innate immune response triggered by the activation of Toll-like receptor 2 in infant mice. In this work, we aimed to evaluate the beneficial effects of D. pigrum 040417 in the context of Streptococcus pneumoniae infection and characterize the role of alveolar macrophages (AMs) in the immunomodulatory properties of this respiratory commensal bacterium. The nasal administration of D. pigrum 040417 to infant mice significantly increased their resistance to pneumococcal infection, differentially modulated respiratory cytokines production, and reduced lung injuries. These effects were associated to the ability of the 040417 strain to modulate AMs function. Depletion of AMs significantly reduced the capacity of the 040417 strain to improve both the reduction of pathogen loads and the protection against lung tissue damage. We also demonstrated that the immunomodulatory properties of D. pigrum are strain-specific, as D. pigrum 030918 was not able to modulate respiratory immunity or to increase the resistance of mice to an S. pneumoniae infection. These findings enhanced our knowledge regarding the immunological mechanisms involved in modulation of respiratory immunity induced by beneficial respiratory commensal bacteria and suggested that particular strains could be used as next-generation probiotics.
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页数:19
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