A Conserved Carboxylesterase Inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana Plants

被引：10
作者：
Guo, Song [1 ,2 ]
Wong, Sek-Man [1 ,2 ,3 ]
机构：
[1] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore
[2] Natl Univ Singapore, Suzhou Res Inst, Suzhou 215123, Peoples R China
[3] Temasek Life Sci Lab, 1 Res Link, Singapore 117604, Singapore
来源：
VIRUSES-BASEL | 2020年 / 12卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Carboxylesterase; Tobacco mosaic virus; host resistance; coat protein; HYPERSENSITIVE RESPONSE; DISEASE RESISTANCE; HELICASE DOMAIN; CELL-DEATH; GENE; ESTERASE; ARABIDOPSIS; EXPRESSION; PROTEINS; CLONING;
D O I：
10.3390/v12020195
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A carboxylesterase (CXE) or carboxylic-ester hydrolase is an enzyme that catalyzes carboxylic ester and water into alcohol and carboxylate. In plants, CXEs have been implicated in defense, development, and secondary metabolism. We discovered a new CXE gene in Nicotiana benthamiana that is related to virus resistance. The transcriptional level of NbCXE expression was significantly increased after Tobacco mosaic virus (TMV) infection. Transient over-expression of NbCXE inhibited TMV accumulation in N. benthamiana plants. Conversely, when the NbCXE gene was silenced with a Tobacco rattle virus (TRV)-based gene silencing system, TMV RNA accumulation was increased in NbCXE-silenced plants after infection. NbCXE protein was shown to interact with TMV coat protein (CP) in vitro. Additionally, the expressions of host defense-related genes were increased in transient NbCXE-overexpressed plants but decreased in NbCXE silenced N. benthamiana plants. In summary, our study showed that NbCXE is a novel resistance-related gene involved in host defense responses against TMV infection.
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页数：11
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