DNA Damage during Meiosis Induces Chromatin Remodeling and Synaptonemal Complex Disassembly

被引:35
|
作者
Couteau, Florence [1 ]
Zetka, Monique [1 ]
机构
[1] McGill Univ, Dept Biol, Montreal, PQ H3A 1B1, Canada
基金
加拿大健康研究院;
关键词
DOUBLE-STRAND BREAKS; C-ELEGANS; MEIOTIC RECOMBINATION; CROSSING-OVER; HOMOLOG ALIGNMENT; CHIASMA FORMATION; SYNAPSIS; PROTEINS; PROPHASE; REPAIR;
D O I
10.1016/j.devcel.2011.01.015
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
DNA damage to the germline genome must be accurately repaired to ensure transmission of intact genetic information to following generations. Meiosis presents challenges to the DNA damage response (DDR) because it universally requires changes to chromosome structure that can affect DNA repair outcomes. We report the existence of a meiotic DDR at chromosome axes that results in chromatin remodeling, synaptonemal complex disassembly, and axis separation in response to irradiation at late pachytene stages in C. elegans. The axis component HTP-3 is required for germline acquisition of H2AacK5, an axis-specific chromatin mark that is DNA damage responsive. Irradiated wild-types show reduction of H2AacK5 and axis separation that are dependent on the acetyltransferase MYS-1/TIP60. Restoration of H2AacK5 levels requires ATM-1 kinase and correlates with resynapsis. We propose that the meiotic DDR involves early chromatin remodeling at chromosome axes to dismantle structures promoting interhomolog recombination and facilitate efficient nonhomolog-based repair before pachytene exit.
引用
收藏
页码:353 / 363
页数:11
相关论文
共 50 条
  • [21] Mutations in the Chromosomal Passenger Complex and the Condensin Complex Differentially Affect Synaptonemal Complex Disassembly and Metaphase I Configuration in Drosophila Female Meiosis
    Resnick, Tamar D.
    Dej, Kimberley J.
    Xiang, Youbin
    Hawley, R. Scott
    Ahn, Caroline
    Orr-Weaver, Terry L.
    GENETICS, 2009, 181 (03) : 875 - 887
  • [22] Rsf-1, a Chromatin Remodeling Protein, Induces DNA Damage and Promotes Genomic Instability
    Sheu, Jim Jinn-Chyuan
    Guan, Bin
    Choi, Jung-Hye
    Lin, Athena
    Lee, Chia-Huei
    Hsiao, Yi-Ting
    Wang, Tian-Li
    Tsai, Fuu-Jen
    Shih, Ie-Ming
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (49) : 38260 - 38269
  • [23] The Arabidopsis SWR1 Chromatin-Remodeling Complex Is Important for DNA Repair, Somatic Recombination, and Meiosis
    Rosa, Marisa
    Von Harder, Mona
    Cigliano, Riccardo Aiese
    Schloegelhofer, Peter
    Scheid, Ortrun Mittelsten
    PLANT CELL, 2013, 25 (06): : 1990 - 2001
  • [24] Yeast polyubiquitin unit regulates synaptonemal complex formation and recombination during meiosis
    Min-Kyung Jo
    Kiwon Rhee
    Keun Pil Kim
    Soogil Hong
    Journal of Microbiology, 2022, 60 : 705 - 714
  • [25] ISWI chromatin remodeling complexes in the DNA damage response
    Aydin, Ozge Z.
    Vermeulen, Wim
    Lans, Hannes
    CELL CYCLE, 2014, 13 (19) : 3016 - 3025
  • [26] Interplay between Synaptonemal Complex, Homologous Recombination, and Centromeres during Mammalian Meiosis
    Qiao, Huanyu
    Chen, Jefferson K.
    Reynolds, April
    Hoog, Christer
    Paddy, Michael
    Hunter, Neil
    PLOS GENETICS, 2012, 8 (06):
  • [27] Organization and Behavior of the Synaptonemal Complex during Achiasmatic Meiosis of Four Buthid Scorpions
    Schneider, Marielle C.
    Mattos, Viviane F.
    Carvalho, Leonardo S.
    Cella, Doralice M.
    CYTOGENETIC AND GENOME RESEARCH, 2014, 144 (04) : 341 - 347
  • [28] Yeast polyubiquitin unit regulates synaptonemal complex formation and recombination during meiosis
    Jo, Min-Kyung
    Rhee, Kiwon
    Kim, Keun Pil
    Hong, Soogil
    JOURNAL OF MICROBIOLOGY, 2022, 60 (07) : 705 - 714
  • [29] DNA translocation and nucleosome remodeling assays by the RSC chromatin remodeling complex
    Wittmeyer, J
    Saha, A
    Cairns, B
    CHROMATIN AND CHROMATIN REMODELING ENZYMES, PT C, 2004, 377 : 322 - 343
  • [30] Assays for chromatin remodeling during DNA repair
    Moggs, JG
    Almouzni, G
    CHROMATIN, 1999, 304 : 333 - 351