Visnagin prevents isoproterenol-induced myocardial injury by attenuating oxidative stress and inflammation and upregulating Nrf2 signaling in rats

被引：32

作者：

Abukhalil, Mohammad H. ^{[1
,2
]}

Hussein, Omnia E. ^{[3
]}

Aladaileh, Saleem H. ^{[2
,4
]}

Althunibat, Osama Y. ^{[2
]}

Al-Amarat, Wesam ^{[5
]}

Saghir, Sultan A. ^{[2
]}

Alfwuaires, Manal A. ^{[6
]}

Algefare, Abdulmohsen, I ^{[6
]}

Alanazi, Khalid M. ^{[7
]}

Al-Swailmi, Farhan K. ^{[4
]}

Kamel, Emadeldin M. ^{[8
]}

Mahmoud, Ayman M. ^{[3
,9
]}

机构：

[1] Al Hussein Bin Talal Univ, Fac Sci, Dept Biol, Maan 71111, Jordan

[2] Al Hussein Bin Talal Univ, Princess Aisha Bint Al Hussein Fac Nursing & Hlth, Dept Med Anal, Maan, Jordan

[3] Beni Suef Univ, Fac Sci, Zool Dept, Physiol Div, Salah Salim St, Bani Suwayf 62514, Egypt

[4] Univ Hafr Al Batin, Coll Pharm, Dept Pharm Practice, Hafar al Batin, Saudi Arabia

[5] Al Balqa Appl Univ, Al Karak Univ Coll, Dept Med Support, As, Jordan

[6] King Faisal Univ, Fac Sci, Dept Biol Sci, Al Hasa, Saudi Arabia

[7] King Saud Univ, Coll Sci, Zool Dept, Riyadh, Saudi Arabia

[8] Beni Suef Univ, Fac Sci, Chem Dept, Bani Suwayf, Egypt

[9] Beni Suef Univ, Res Inst Med & Aromat Plants, Biotechnol Dept, Bani Suwayf, Egypt

来源：

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY | 2021年 / 35卷 / 11期

关键词：

isoproterenol; myocardial infarction; Nrf2; oxidative stress; visnagin; ACTIVATED RECEPTOR-GAMMA; PROTECTIVE ROLE; CELL-DEATH; INFARCTION; HEART; FAILURE; AGONIST; MARKERS; GENE; BETA;

D O I：

10.1002/jbt.22906

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxidative tissue injury and inflammatory responses play major roles in cardiovascular diseases and heart failure. Visnagin (VIS) is a natural bioactive component of Ammi visnaga, with promising radical scavenging and anti-inflammatory activities. This study explored the protective effect of VIS against isoproterenol (ISO)-induced acute myocardial injury and oxidative stress in rats. VIS was supplemented for 14 days, and the rats received ISO (100 mg/kg) twice at an interval of 24 h. ISO-induced myocardial injury was characterized by elevated serum CK-MB, LDH, and troponin-I associated with increased heart weight and several histopathological changes. ISO increased reactive oxygen species (ROS), malondialdehyde (MDA), NF-kappa B p65, TNF-alpha, IL-6, and decreased glutathione and antioxidant enzymes in rats' hearts. VIS prevented myocardial injury and ameliorated the cardiac function markers, ROS, MDA, NF-kappa B p65, and pro-inflammatory cytokines in ISO-intoxicated rats. In addition, VIS decreased Bax mRNA and caspases, and upregulated Nrf2, HO-1, Bcl-2, and PPAR gamma. Molecular docking simulations revealed the binding method of VIS to NF-kappa B, Keap1, and PPAR gamma. In conclusion, VIS protects against ISO-induced acute myocardial injury by attenuating oxidative tissue injury and reducing key inflammatory and apoptosis markers. In vivo and in silico results showed that activation of Nrf2/HO-1 signaling and PPAR gamma mediates the cardioprotective effect of VIS.

引用

页数：13

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