Profiles of microRNA in aqueous humor of normal tension glaucoma patients using RNA sequencing

被引:18
作者
Seong, Hyemin [1 ]
Cho, Hyun-kyung [2 ,3 ]
Kee, Changwon [4 ]
Song, Dae Hyun [3 ,5 ]
Cho, Min-Chul [3 ,6 ]
Kang, Sang Soo [1 ,3 ]
机构
[1] Gyeongsang Natl Univ, Dept Anat & Convergence Med Sci, Jinju, South Korea
[2] Gyeongsang Natl Univ, Sch Med, Changwon Hosp, Dept Ophthalmol, Chang Won, South Korea
[3] Gyeongsang Natl Univ, Sch Med, Inst Hlth Sci, Jinju, South Korea
[4] Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Dept Ophthalmol, Seoul, South Korea
[5] Gyeongsang Natl Univ, Changwon Hosp, Dept Pathol, Chang Won, South Korea
[6] Gyeongsang Natl Univ, Gyeongsang Natl Univ Hosp, Coll Med, Dept Lab Med, Jinju, South Korea
基金
新加坡国家研究基金会;
关键词
OPEN-ANGLE GLAUCOMA; LAMINA-CRIBROSA THICKNESS; OPTIC-NERVE HEAD; MESSENGER-RNA; DIFFERENTIATION; EXPRESSION; MIR-375; GROWTH; IDENTIFICATION; PROGRESSION;
D O I
10.1038/s41598-021-98278-0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We aimed to identify and compare microRNAs (miRNAs) from individual aqueous humor samples between normal-tension glaucoma (NTG) patients and normal controls. Aqueous humor (80 to 120 mu l) was collected before cataract surgery. Six stable NTG patients and seven age-matched controls were included in the final analysis. RNA sequencing was conducted for RNA samples extracted from the 13 aqueous humor samples, and bioinformatics analysis was employed for the miRNA targets and related pathways. Two hundred and twenty-eight discrete miRNAs were detected in the aqueous humor and consistently expressed in all samples. Eight significantly upregulated miRNAs were found in the NTG patients compared to the controls (fold-change > 2, p < 0.05). They were hsa-let-7a-5p, hsa-let-7c-5p, hsa-let-7f-5p, hsa-miR-192-5p, hsa-miR-10a-5p, hsa-miR-10b-5p, hsa-miR-375, and hsa-miR-143-3p. These miRNAs were predicted to be associated with the biological processes of apoptosis, autophagy, neurogenesis, and aging in the gene ontology categories. The related Kyoto encyclopedia of genes and genomes pathways were extracellular matrix-receptor interaction, mucin-type O-glycan biosynthesis, biotin metabolism, and signaling pathways regulating the pluripotency of stem cells. The differentially expressed miRNA in the NTG samples compared to the controls suggest the possible roles of miRNA in the pathogenesis of NTG. The underlying miRNA-associated pathways further imply novel targets for the pathogenesis of NTG.
引用
收藏
页数:12
相关论文
共 82 条
[1]   miR-375 Inhibits Differentiation of Neurites by Lowering HuD Levels [J].
Abdelmohsen, Kotb ;
Hutchison, Emmette R. ;
Lee, Eun Kyung ;
Kuwano, Yuki ;
Kim, Mihee M. ;
Masuda, Kiyoshi ;
Srikantan, Subramanya ;
Subaran, Sarah S. ;
Marasa, Bernard S. ;
Mattson, Mark P. ;
Gorospe, Myriam .
MOLECULAR AND CELLULAR BIOLOGY, 2010, 30 (17) :4197-4210
[2]   Molecular biomarkers in primary open-angle glaucoma: from noninvasive to invasive [J].
Agnifili, Luca ;
Pieragostino, Damiana ;
Mastropasqua, Alessandra ;
Fasanella, Vincenzo ;
Brescia, Lorenza ;
Tosi, Gian Marco ;
Sacchetta, Paolo ;
Mastropasqua, Leonardo .
NEW TRENDS IN BASIC AND CLINICAL RESEARCH OF GLAUCOMA: A NEURODEGENERATIVE DISEASE OF THE VISUAL SYSTEM, PT B, 2015, 221 :1-32
[3]   A major marker for normal tension glaucoma:: association with polymorphisms in the OPA1 gene [J].
Aung, T ;
Ocaka, L ;
Ebenezer, ND ;
Morris, AG ;
Krawczak, M ;
Thiselton, DL ;
Alexander, C ;
Votruba, M ;
Brice, G ;
Child, AH ;
Francis, PJ ;
Hitchings, RA ;
Lehmann, OJ ;
Bhattacharya, SS .
HUMAN GENETICS, 2002, 110 (01) :52-56
[4]   MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].
Bartel, David P. .
CELL, 2007, 131 (04) :11-29
[5]   miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA [J].
Bellon, Anais ;
Iyer, Archana ;
Bridi, Simone ;
Lee, Flora C. Y. ;
Ovando-Vazquez, Cesare ;
Corradi, Eloina ;
Longhi, Sara ;
Roccuzzo, Michela ;
Strohbuecker, Stephanie ;
Naik, Sindhu ;
Sarkies, Peter ;
Miska, Eric ;
Abreu-Goodger, Cei ;
Holt, Christine E. ;
Baudet, Marie-Laure .
CELL REPORTS, 2017, 18 (05) :1171-1186
[6]   CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].
BENJAMINI, Y ;
HOCHBERG, Y .
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300
[7]   Spontaneous activity and stretch-induced contractile differentiation are reduced in vascular smooth muscle of miR-143/145 knockout mice [J].
Bhattachariya, A. ;
Dahan, D. ;
Ekman, M. ;
Boettger, T. ;
Braun, T. ;
Sward, K. ;
Hellstrand, P. ;
Albinsson, S. .
ACTA PHYSIOLOGICA, 2015, 215 (03) :133-143
[8]   MiR-375 is Essential for Human Spinal Motor Neuron Development and May Be Involved in Motor Neuron Degeneration [J].
Bhinge, Akshay ;
Namboori, Seema C. ;
Bithell, Angela ;
Soldati, Chiara ;
Buckley, Noel J. ;
Stanton, Lawrence W. .
STEM CELLS, 2016, 34 (01) :124-134
[9]   The genetics of primary open angle glaucoma [J].
Booth, A ;
Churchill, A ;
Anwar, R ;
Menage, M ;
Markham, A .
BRITISH JOURNAL OF OPHTHALMOLOGY, 1997, 81 (05) :409-414
[10]   The role of let-7 in cell differentiation and cancer [J].
Boyerinas, Benjamin ;
Park, Sun-Mi ;
Hau, Annika ;
Murmann, Andrea E. ;
Peter, Marcus E. .
ENDOCRINE-RELATED CANCER, 2010, 17 (01) :F19-F36