Insulin and insulin-like growth factor-1 stimulate dephosphorylation of paxillin in parallel with focal adhesion kinase

被引：24

作者：

Konstantopoulos, N ^{[1
]}

Clark, S ^{[1
]}

机构：

[1] UNIV MELBOURNE,DEPT MED,PARKVILLE,VIC 3050,AUSTRALIA

来源：

BIOCHEMICAL JOURNAL | 1996年 / 314卷

关键词：

D O I：

10.1042/bj3140387

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Paxillin and focal adhesion kinase (pp125(FAK)) co-localize in focal adhesions; recently insulin has been shown to stimulate the dephosphorylation of pp 125(FAK), however, its effect on paxillin is unknown. We show that insulin and IGF-1 can stimulate the dephosphorylation of paxillin in CHOT (overexpress human insulin receptors) and CHO Delta CT69 (overexpress insulin receptors lacking C-terminal 69 amino acids) cells. Furthermore, the insulin-receptor C-terminus is not needed for either insulin or IGF-1 to stimulate paxillin or pp125(FAK) dephosphorylation in the CHO (Chinese-hamster ovary) cell lines used.

引用

页码：387 / 390

页数：4

共 20 条

[1] CHARACTERIZATION OF TYROSINE PHOSPHORYLATION OF PAXILLIN IN-VITRO BY FOCAL ADHESION KINASE [J].

BELLIS, SL ;

MILLER, JT ;

TURNER, CE .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (29) :17437-17441

[2] TYROSINE PHOSPHORYLATION OF PAXILLIN AND PP125(FAK) ACCOMPANIES CELL-ADHESION TO EXTRACELLULAR-MATRIX - A ROLE IN CYTOSKELETAL ASSEMBLY [J].

BURRIDGE, K ;

TURNER, CE ;

ROMER, LH .

JOURNAL OF CELL BIOLOGY, 1992, 119 (04) :893-903

[3] INSULIN ACTION AND THE INSULIN SIGNALING NETWORK [J].

CHEATHAM, B ;

KAHN, CR .

ENDOCRINE REVIEWS, 1995, 16 (02) :117-142

[4]

CLARK EA, 1995, NATURE, V268, P233

[5] CHANGES IN INSULIN-RECEPTOR STRUCTURE ASSOCIATED WITH TRYPSIN-INDUCED ACTIVATION OF THE RECEPTOR TYROSINE KINASE [J].

CLARK, S ;

ECKARDT, G ;

SIDDLE, K ;

HARRISON, LC .

BIOCHEMICAL JOURNAL, 1991, 276 :27-33

[6] INSULIN-RECEPTOR AUTOPHOSPHORYLATION AND EXOGENOUS SUBSTRATE PHOSPHORYLATION - ROLE OF RECEPTOR C-TERMINUS AND EFFECTS OF MILD REDUCTION [J].

CLARK, S ;

KONSTANTOPOULOS, N .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 200 (01) :330-337

[7]

FARIA TN, 1994, J BIOL CHEM, V269, P13922

[8] PAXILLIN, A TYROSINE-PHOSPHORYLATED FOCAL ADHESION-ASSOCIATED PROTEIN BINDS TO THE CARBOXYL-TERMINAL DOMAIN OF FOCAL ADHESION KINASE [J].

HILDEBRAND, JD ;

SCHALLER, MD ;

PARSONS, JT .

MOLECULAR BIOLOGY OF THE CELL, 1995, 6 (06) :637-647

[9] EXPRESSION OF ATYPICAL AND CLASSICAL INSULIN-RECEPTORS IN CHINESE HAMSTER OVARY CELLS TRANSFECTED WITH CLONED CDNA FOR THE HUMAN INSULIN-RECEPTOR [J].

JONAS, HA ;

ECKARDT, GS ;

CLARK, S .

ENDOCRINOLOGY, 1990, 127 (03) :1301-1309

[10]

KADOWAKI T, 1986, J BIOL CHEM, V261, P6141

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