Determination of myrislignan levels in BALB/c mouse plasma by LC-MS/MS and a comparison of its pharmacokinetics after oral and intraperitoneal administration

被引:8
作者
Zhang, Jili [1 ,2 ,3 ]
Si, Hongfei [4 ]
Sun, Jichao [5 ]
Lv, Kun [6 ]
Yan, Biqing [1 ]
Li, Bing [3 ]
Zhou, Xuzheng [3 ]
Zhang, Jiyu [3 ,7 ]
机构
[1] Ningbo Univ, Affiliated Hosp, Med Sch, Intens Care Unit, Ningbo, Zhejiang, Peoples R China
[2] Ningbo Univ, Sch Med, Ningbo, Zhejiang, Peoples R China
[3] Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Lanzhou, Gansu, Peoples R China
[4] Jinan Univ, Coll Pharm, Guangzhou, Guangdong, Peoples R China
[5] Northeast Agr Univ, Coll Vet Med, Harbin, Heilongjiang, Peoples R China
[6] Ningbo Univ, Sch Business, Ningbo, Zhejiang, Peoples R China
[7] Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Key Lab Vet Pharmaceut Dev, Lanzhou 730050, Gansu, Peoples R China
关键词
Myrislignan; Pharmacokinetics; LC-MS; MS; Mouse; Dehydrodiisoeugenol; Bioavailability; MYRISTICA-FRAGRANS; RAT PLASMA; NUTMEG; NEOLIGNANS; SEEDS;
D O I
10.1186/s12917-021-02990-y
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Background Myrislignan is a natural product from Myristica sp. with diverse pharmacological activities. Recently, the anti-Toxoplasma gondii (T. gondii) activity of myrislignan has been proposed, and in vivo studies of its pharmacokinetics in BALB/c mice are necessary to further evaluate the clinical effects of myrislignan. Results In this study, a sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to quantify myrislignan levels in mouse plasma using dehydrodiisoeugenol as an internal standard (IS) in positive ion mode. Chromatographic separation of the analytes was achieved using an ACE Ultracore Super C18 analytical column (2.5 mu m, 2.1 x 50 mm) at 30 degrees C. A gradient mobile phase consisting of water (0.1 % formic acid) and acetonitrile (0.1 % formic acid) was delivered at a flow rate of 0.4 mL/min. Myrislignan and the IS eluted at 1.42 and 1.71 min, respectively. A good excellent linear response across the concentration range of 1-1000 ng/mL was achieved (r(2) = 0.9973). The lower limit of quantification (LLOQ) was 1 ng/mL, and the inter- and intra-day accuracy and precision of the method showed relative standard deviations (RSDs) less than 10 %. The method was applied to examine the pharmacokinetics of myrislignan in mouse plasma following a single oral administration of 200 mg/kg or intraperitoneal administration of 50 mg/kg myrislignan, and the bioavailability (F) of orally administered myrislignan was only 1.97 % of the bioavailability of intraperitoneally administered myrislignan. Conclusions A rapid and sensitive LC-MS/MS method has been was developed, validated and successfully used to determine myrislignan levels in mice after oral or intraperitoneal administration. This study is the first to report the pharmacokinetic parameters of myrislignan in mice and to compare its pharmacokinetics after oral and intraperitoneal administration, which will be useful for further research on the administration of myrislignan in animals and humans.
引用
收藏
页数:10
相关论文
共 50 条
  • [41] LC-MS/MS analysis of doxycycline residues in chicken tissues after oral administration
    Gajda, Anna
    Posyniak, Andrzej
    Tomczyk, Grzegorz
    [J]. BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY, 2014, 58 (04) : 573 - 579
  • [42] Simultaneous determination of glaucocalyxin A and glaucocalyxin B in rat plasma by LC-MS/MS and its application to a pharmacokinetic study after oral administration of Rabdosia japonica extract
    Huang, Weili
    Guan, Xiaohui
    Lv, Yongchen
    [J]. BIOMEDICAL CHROMATOGRAPHY, 2018, 32 (02)
  • [43] Determination of Momelotinib in Rat Plasma by LC-MS/MS: Application to Pharmacokinetics Study
    Babu, Bysani Suresh
    Baby, Nalanda Revu
    Mondal, Sumanta
    [J]. INTERNATIONAL JOURNAL OF PHARMACEUTICAL INVESTIGATION, 2025, 15 (02) : 388 - 396
  • [44] Development and validation of an LC-MS/ MS method for the determination of hinokiflavone in rat plasma and its application to a pharmacokinetic study
    Yin, Ruofeng
    Xiong, Kun
    Wen, Simin
    Wang, Yuanyi
    Xu, Feng
    [J]. BIOMEDICAL CHROMATOGRAPHY, 2017, 31 (03)
  • [45] Determination of ginsenoside Rh3 in rat plasma by LC-MS/MS and its application to a pharmacokinetic study
    Yang, Ting
    Xu, Wenwu
    Wei, Xiyu
    Zhang, Zhenzhen
    Sun, Yue
    Liu, Houru
    Yu, Peihua
    Li, Wei
    Yu, Dehong
    [J]. BIOMEDICAL CHROMATOGRAPHY, 2022, 36 (02)
  • [46] LC-MS/MS method for the determination of agomelatine in human plasma and its application to a pharmacokinetic study
    Wang, Xiaolin
    Zhang, Dan
    Liu, Man
    Zhao, Hongna
    Du, Aihua
    Meng, Lingjie
    Liu, Huichen
    [J]. BIOMEDICAL CHROMATOGRAPHY, 2014, 28 (02) : 218 - 222
  • [47] Development and Validation of LC-MS/MS Method for Determination of Ondansetron in rat Plasma and its Application
    Pang, Lingxia
    Wang, Qing
    Wang, Youpei
    Zheng, Meiqin
    Wu, Haiya
    Wang, Zhiyi
    [J]. LATIN AMERICAN JOURNAL OF PHARMACY, 2012, 31 (02): : 305 - 309
  • [48] A simplified LC-MS/MS approach for simultaneous quantification and pharmacokinetics of five compounds in rats following oral administration of Gastrodia elata extract
    Dong, Jiajia
    Ji, De
    Su, Lianlin
    Zhang, Fengyang
    Tong, Huangjin
    Mao, Chunqin
    Lu, Tulin
    [J]. JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY, 2020, 11 (01)
  • [49] Determination of an isonicotinylhydrazide derivative, a novel positive inotropic compound, in mouse plasma by LC-MS/MS and its application to a pharmacokinetics study
    Hui, Renjie
    Gao, Yueying
    Liu, Jingyi
    Liu, Haili
    Tang, Chunlei
    Feng, Bainian
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2019, 165 : 12 - 17
  • [50] Determination of 3-α-hydroxytibolone in human plasma by LC-MS/MS: application for a pharmacokinetic study after administration of a tibolone formulation
    de Santana e Silva Cardoso, Fabiana Fernandes
    Cesar, Isabela Costa
    Mundim, Iram Moreira
    Teixeira, Leonardo de Souza
    da Silva, Enikson Pontes
    Bonfim, Ricardo Rodrigues
    Gomes, Sandro Antonio
    Ferreira, Denys Pires
    Batista Lopes, Aderimar Rogerio
    Pascoal, Helifas Duarte
    de Souza, Weidson Carlo
    Pianetti, Gerson Antonio
    [J]. BIOMEDICAL CHROMATOGRAPHY, 2013, 27 (11) : 1457 - 1462