HLA-A towards a high-resolution DNA typing

被引：4
作者：
Scheltinga, SA
Williams, F
van der Zwan, AW
Rozemuller, EH
Middleton, D
Tilanus, MGJ
机构：
[1] Univ Utrecht Hosp, Dept Pathol, NL-3508 GA Utrecht, Netherlands
[2] Belfast City Hosp, No Ireland Tissue Typing Lab, Belfast BT9 7AD, Antrim, North Ireland
[3] Queens Univ Belfast, Belfast BT7 1NN, Antrim, North Ireland
[4] Univ Ulster, Belfast, Antrim, North Ireland
来源：
TISSUE ANTIGENS | 1998年 / 51卷 / 05期
关键词：
HLA-A; sequencing-based typing; sequence-specific oligonucleotide probes;
D O I：
10.1111/j.1399-0039.1998.tb02990.x
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Sequencing-based typing (SBT) and sequence-specific oligonucleotide probing (PCR-SSOP) are DNA-based typing approaches to identify HLA-A alleles. In this study PCR-SSOP SET have been evaluated and considered to reach a high-resolution typing. Based upon serological typing, 32 genomic samples were typed by SET and PCR-SSOP. Three main clusters of resolution could be defined. The advantage of the PCR-SSOP approach is the possibility to type numerous samples in a short time. SET minimizes the number of ambiguous heterozygous combinations and often allows direct detection and identification of new alleles.
引用
收藏
页码：549 / 552
页数：4
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