Multidrug resistance-1 gene C3435T single nucleotide polymorphism in OSAS patients

Aim: Obstructive sleep apnea syndrome (OSAS) is characterized by decreased airflow (hypopnea) or complete interruption (apnea) and is characterized by airway narrowing, decreased oxyhemoglobin saturation, hypercapnia, and hyperventilation associated therewith. It is known that genetic factors play role in the pathogenesis of OSAS. Multi-drug resistant-1 (MDR-1) gene products have a role in the pathogenesis of several diseases related to oxidative stress. We aimed to evaluate the frequency of MDR-1 gene C/T polymorphism in patients with OSAS. Material and Method: This study was performed on 43 OSAS and 23 healthy individuals. Blood samples from each individual were collected in tubes with ethylenediaminetetraacetic add (EDTA). DNA was extracted from the blood samples. The MDR-1 gene polymorphism was detected by polymerase chain reaction (PCR) and enzyme digestion techniques. Results: The frequencies of MDR-1 genotypes were found 27.9% for CC, 41.9% for CT and 30.2% for TT in the OSAS group and 17.4% for CC, 65.2% for CT and 17.4% for TT in the control group. The distribution of MDR-1 gene C alleles was found to be 48.8% in COPD group and 50.0% in the control group; T alleles were found to be 51.2% in OSAS group and 50.0% in the control group. There was no statistically significant difference observed between the groups for genotype and allele frequency of MDR-1 gene (p>0.05). Discussion: We believe these results can be useful for large-scale population genetic research considering the frequency of the MDR-1 gene variation in OSAS patients in the Turkish population.