G-Quadruplex-Based DNAzymes Aptasensor for the Amplified Electrochemical Detection of Thrombin

被引：30

作者：

Shen, Bijun ^{[1
]}

Wang, Qi ^{[1
]}

Zhu, Dan ^{[1
]}

Luo, Juanjuan ^{[1
]}

Cheng, Guifang ^{[1
]}

He, Pingang ^{[1
]}

Fang, Yuzhi ^{[1
]}

机构：

[1] E China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China

来源：

ELECTROANALYSIS | 2010年 / 22卷 / 24期

关键词：

DNAzyme; Aptasensor; Thrombin; G-Quadruplex; Electrochemistry; COLORIMETRIC DETECTION; DNA; APTAMER; NANOPARTICLES; LABEL; AMPLIFICATION; BIOSENSOR; ELECTRODE; FILM;

D O I：

10.1002/elan.201000360

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel G-quadruplex-based DNAzymes aptasensor for the amplified electrochemical detection of thrombin has been described. The aptasensor utilized a combination of hemin and guanine-rich thrombin-binding aptamer (TBA) to form horseradish peroxidase (HRP)-mimicking DNAzymes with peroxidase catalytic activity. In the presence of thrombin, the enzyme activity could be extensively promoted, thereby providing the amplified electrochemical read-out signals for detecting thrombin. This aptasensor exhibited high sensitivity and selectivity for thrombin determination, which enabled the analysis of thrombin with a detection limit of 6 x 10(-11) M. On the basis of results, this method could have broad applications in the detection of proteins and other biomolecules.

引用

页码：2985 / 2990

页数：6

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