Characterization, bioactivities, and rheological properties of exopolysaccharide produced by novel probiotic Lactobacillus plantarum C70 isolated from camel milk

被引：116

作者：

Ayyash, Mutamed ^{[1
]}

Abu-Jdayil, Basim ^{[2
]}

Itsaranuwat, Pariyaporn ^{[3
]}

Galiwango, Emmanuel ^{[2
]}

Tamiello-Rosa, Camila ^{[1
]}

Abdullah, Hassan ^{[1
]}

Esposito, Gennaro ^{[4
]}

Hunashal, Yamanappa ^{[4
]}

Obaid, Reyad S. ^{[5
]}

Hamed, Fathalla ^{[6
]}

机构：

[1] UAEU, Coll Food & Agr, Dept Food Nutr & Hlth, Al Ain, U Arab Emirates

[2] UAEU, Coll Engn, Chem & Petr Engn Dept, Al Ain, U Arab Emirates

[3] Mahasarakham Univ, Fac Technol, Dept Biotechnol, Maha Sarakham 44150, Thailand

[4] New York Univ Abu Dhabi, Sci Div, NYUAD Campus, Abu Dhabi, U Arab Emirates

[5] Univ Sharjah, Dept Clin Nutr & Dietet, Sharjah, U Arab Emirates

[6] UAEU, Coll Sci, Dept Phys, Al Ain, U Arab Emirates

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2020年 / 144卷

关键词：

Exopolysaccharides; L; plantarum; Anticancer; Antidiabetic; Rheological properties; STRUCTURAL-CHARACTERIZATION; ANTIOXIDANT ACTIVITY; ALPHA-AMYLASE; POLYSACCHARIDES; PURIFICATION; ELUCIDATION; INHIBITION; EPS;

D O I：

10.1016/j.ijbiomac.2019.09.171

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Various industries highly regard the functionalities and bioactivities of bacterial polysaccharides. We aimed to characterize the exopolysaccharide (EPS) produced by novel probiotic Lactobacillus plantarum C70 (accession number KX881779) isolated from camel milk and to investigate its bioactivities and rheological properties. EPS-C70 had a weight-average molecular weight (Mw) of 3.8 x 10(5) Da. Arabinose (13.3%), mannose (7.1%), glucose (74.6%), and galactose (5.0%) were the major monosaccharides constituents. EPS-C70 had two endothermic peaks at 76.95 degrees C and 158.76 degrees C corresponding to glass transition (T-g) and melting point (T-m), respectively. Zeta potential and particle size of EPS-C70 were -330.71 mV and 525.5 nm, respectively. DPPH and ABTS of EPS-C70 were 75.91% and 49.42% at 10 mg/mL concentration, respectively. The cytotoxic activities against colon cancer and breast cancer lines were 88.1% and 73.1% at concentration 10 mg/mL, respectively. EPS-C70 exhibited shear-thinning behaviour. Salts and pH values had a significant impact on the rheological properties of EPS-C70. (C) 2019 Elsevier B.V. All rights reserved.

引用

页码：938 / 946

页数：9

共 40 条

[1] Characterization of potential probiotic lactic acid bacteria isolated from camel milk [J].

Abushelaibi, Aisha ;

Al-Mahadin, Suheir ;

El-Tarabily, Khaled ;

Shah, Nagendra P. ;

Ayyash, Mutamed .

LWT-FOOD SCIENCE AND TECHNOLOGY, 2017, 79 :316-325

[2] Characterization of exopolysaccharide produced by Lactobacillus kefiranofaciens ZW3 isolated from Tibet kefir - Part II [J].

Ahmed, Zaheer ;

Wang, Yanping ;

Anjum, Nomana ;

Ahmad, Asif ;

Khan, Salman Tariq .

FOOD HYDROCOLLOIDS, 2013, 30 (01) :343-350

[3] In vitro investigation of bioactivities of solid-state fermented lupin, quinoa and wheat using Lactobacillus spp. [J].

Ayyash, Mutamed ;

Johnson, Stuart K. ;

Liu, Shao-Quan ;

Mesmari, Nouf ;

Dahmani, Shaikhah ;

Al Dhaheri, Ayesha S. ;

Kizhakkayil, Jaleel .

FOOD CHEMISTRY, 2019, 275 :50-58

[4] In vitro investigation of anticancer and ACE-inhibiting activity, α-amylase and α-glucosidase inhibition, and antioxidant activity of camel milk fermented with camel milk probiotic: A comparative study with fermented bovine milk [J].

Ayyash, Mutamed ;

Al-Nuaimi, Amna K. ;

Al-Mahadin, Suheir ;

Liu, Shao-Quan .

FOOD CHEMISTRY, 2018, 239 :588-597

[5] Chemical and rheological properties of polysaccharides from fruit body of Auricularia auricular-judae [J].

Bao, Honghui ;

You, SangGuan ;

Cao, Longkui ;

Zhou, Rui ;

Wang, Qi ;

Cui, Steve W. .

FOOD HYDROCOLLOIDS, 2016, 57 :30-37

[6] Preparation and structural elucidation of a glucomannogalactan from marine fungus Penicillium commune [J].

Chen, Yanli ;

Mao, Wenjun ;

Wang, Junfeng ;

Zhu, Weiming ;

Zhao, Chunqi ;

Li, Na ;

Wang, Chunyan ;

Yan, Mengxia ;

Guo, Tao ;

Liu, Xue .

CARBOHYDRATE POLYMERS, 2013, 97 (02) :293-299

[7] Characterization, antioxidant property and cytoprotection of exopolysaccharide-capped elemental selenium particles synthesized by Bacillus paralicheniformis SR14 [J].

Cheng, Yuanzhi ;

Xiao, Xiao ;

Li, Xiaoxiao ;

Song, Deguang ;

Lu, Zeqing ;

Wang, Fengqin ;

Wang, Yizhen .

CARBOHYDRATE POLYMERS, 2017, 178 :18-26

[8] Rheological, textural and emulsifying properties of an exopolysaccharide produced by Mesorhizobium loti grown on a crude glycerol-based medium [J].

de Oliveira, Joice Miliane ;

Amaral, Sabrina Adamoli ;

Veiga Burkert, Carlos Andre .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2018, 120 :2180-2187

[9] A novel purified Lactobacillus acidophilus 20079 exopolysaccharide, LA-EPS-20079, molecularly regulates both apoptotic and NF-κB inflammatory pathways in human colon cancer [J].

El-Deeb, Nehal M. ;

Yassin, Abdelrahman M. ;

Al-Madboly, Lamiaa A. ;

El-Hawiet, Amr .

MICROBIAL CELL FACTORIES, 2018, 17

[10] Blends and composites of exopolysaccharides; properties and applications: A review [J].

Hussain, Abid ;

Zia, Khalid Mahmood ;

Tabasum, Shazia ;

Noreen, Aqdas ;

Ali, Muhammad ;

Iqbal, Rehana ;

Zuber, Mohammad .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2017, 94 :10-27

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