Inhibition of staurosporine-induced apoptosis of endothelial cells by activated protein C requires protease-activated receptor-1 and endothelial cell protein C receptor

被引：192

作者：

Mosnier, LO ^{[1
]}

Griffin, JH ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA

来源：

BIOCHEMICAL JOURNAL | 2003年 / 373卷

关键词：

activated protein C; apoptosis; endothelial cell protein C receptor (EPCR); protease-activated receptor (PAR);

D O I：

10.1042/BJ20030341

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In a model of staurosporine-induced apoptosis using EAhy926 endothelial cells, inhibition of apoptosis by activated protein C was dose-dependent and required the enzyme's active site, implicating activated protein C-mediated proteolysis. Consistent with this implication, both protease-activated receptor-1 (PAR-1) and endothelial cell protein C receptor (EPCR) were required for the anti-apoptotic effects of activated protein C.

引用

页码：65 / 70

页数：6

共 26 条

[11] MOLECULAR-CLONING AND EXPRESSION OF MURINE AND BOVINE ENDOTHELIAL-CELL PROTEIN C/ACTIVATED PROTEIN-C RECEPTOR (EPCR) - THE STRUCTURAL AND FUNCTIONAL CONSERVATION IN HUMAN, BOVINE, AND MURINE EPCR [J].

FUKUDOME, K ;

ESMON, CT .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (10) :5571-5577

[12]

Gale AJ, 1997, PROTEIN SCI, V6, P132

[13] Activated protein C:: Potential therapy for severe sepsis, thrombosis, and stroke [J].

Griffin, JH ;

Zlokovic, B ;

Fernández, JA .

SEMINARS IN HEMATOLOGY, 2002, 39 (03) :197-205

[14] IMMUNOBLOTTING STUDIES OF THE MOLECULAR-FORMS OF PROTEIN-C IN PLASMA [J].

HEEB, MJ ;

SCHWARZ, HP ;

WHITE, T ;

LAMMLE, B ;

BERRETTINI, M ;

GRIFFIN, JH .

THROMBOSIS RESEARCH, 1988, 52 (01) :33-43

[15] YOPRO-1 PERMITS CYTOFLUOROMETRIC ANALYSIS OF PROGRAMMED CELL-DEATH (APOPTOSIS) WITHOUT INTERFERING WITH CELL VIABILITY [J].

IDZIOREK, T ;

ESTAQUIER, J ;

DEBELS, F ;

AMEISEN, JC .

JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 185 (02) :249-258

[16] Activated protein C prevents endotoxin-induced hypotension in rats by inhibiting excessive production of nitric oxide [J].

Isobe, H ;

Okajima, K ;

Uchiba, M ;

Mizutani, A ;

Harada, N ;

Nagasaki, A ;

Okabe, K .

CIRCULATION, 2001, 104 (10) :1171-1175

[17] Gene expression profile of antithrombotic protein C defines new mechanisms modulating inflammation and apoptosis [J].

Joyce, DE ;

Gelbert, L ;

Ciaccia, A ;

DeHoff, B ;

Grinnell, BW .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (14) :11199-11203

[18] Plasmin desensitization of the PARI thrombin receptor: Kinetics, sites of truncation, and implications for thrombolytic therapy [J].

Kuliopulos, A ;

Covic, L ;

Seeley, SK ;

Sheridan, PJ ;

Helin, J ;

Costello, CE .

BIOCHEMISTRY, 1999, 38 (14) :4572-4585

[19] The crystal structure of the endothelial protein C receptor and a bound phospholipid [J].

Oganesyan, V ;

Oganesyan, N ;

Terzyan, S ;

Qu, DF ;

Dauter, Z ;

Esmon, NL ;

Esmon, CT .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (28) :24851-24854

[20] The endothelial cell protein C receptor - Inhibition of activated protein C anticoagulant function without modulation of reaction with proteinase inhibitors [J].

Regan, LM ;

StearnsKurosawa, DJ ;

Kurosawa, S ;

Mollica, J ;

Fukudome, K ;

Esmon, CT .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (29) :17499-17503

← 1 2 3 →